Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 5, Problem 10P
Interpretation Introduction
Interpretation:
The distance between the given PAM sequences is to be stated.
Concept introduction:
The full form of PAM sequence is Protospacer Adjacent Motif sequence. It is a small DNA sequence that contains approximately six base pairs lengthwise. The CRISPR-Cas9 targets the DNA region of PAM sequence for cleavage.
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Cynt
Classifying mutations
A certain section of the coding (sense) strand of some DNA looks like this:
$-ATGTATATCTCCAGTTAG-3"
It's known that a very small gene is contained in this section.
Classify each of the possible mutations of this DNA shown in the table below.
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5- ATGTATCATCTCCAGTTAG-3'
S-ATGTATATCTCCAGTTAG-3
5- ATGTATATATCCAGTTAG-3'
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The RNA polymerase from bacteriophage T7 diff ers structurally from prokaryotic and eukaryotic RNAPs and is extremely specifi c for its own promoter. Why do these properties make T7 RNAP useful in experiments with recombinant DNA?
. The double-stranded circular DNA molecule thatforms the genome of the SV40 tumor virus can be denatured into single-stranded DNA molecules. Becausethe base composition of the two strands differs, thestrands can be separated on the basis of their densityinto two strands designated W(atson) and C(rick). When each of the purified preparations of the single strands was mixed with mRNA from cells infectedwith the virus, hybrids were formed between the RNAand DNA. Closer analysis of these hybridizationsshowed that RNAs that hybridized with the W preparation were different from RNAs that hybridized withthe C preparation. What does this tell you about thetranscription templates for the different classes ofRNAs?
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