Concept explainers
(a) To calculate:
The number of superhelical turns in an underwound plasmid pBR322 (4362 base pairs) having -0.05 superhelix density (s).
Concept Introduction:
DNA (deoxyribonucleic acid) supercoiling is the under-or-over winding of a strand DNA double helix. DNA supercoiling is essential for many biological processes, such as DNA compacting, possibly genetic expression, and DNA
(b) To determine:
Number of superhelical turns in the same plasmid if the molecule is overwound having superhelix density of 0.05.
Concept Introduction:
DNA (deoxyribonucleic acid) supercoiling is the under-or-over winding of a strand DNA double helix. DNA supercoiling is essential for many biological processes, such as DNA compacting, possibly genetic expression, and DNA metabolism.
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Biochemistry: Concepts and Connections (2nd Edition)
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