Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 3, Problem 2R
Summary Introduction

Introduction:

Microscopes are generally used to magnify microorganisms and other smaller objects. In order to visualize microorganisms, the right kind of microscope has to be used. The choice can be made based on their size as well as the nature of the specimen.

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Staining of cells is often performed to enhance images acquired by brightfield microscopy. Phase-contrast microscopy does not require cell staining. Why is this advantageous?
A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…
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