What are the differences and similarities between Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Please explain in detail.
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What are the differences and similarities between Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Please explain in detail.
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- 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?Define scanning electron microscope (SEM)What type of microscopy is frequently used now for determining the cellular location of a molecule of interest? Briefly describe the basis of this technique.
- What is phase contrast? Give examples of phase contrast in optical microscopy.Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?why is microtome important in microscopy?