Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 3, Problem 3A

Why isn’t the Gram stain used on acid-fast bacteria? If you did Gram stain acid-fast bacteria, what would their Gram reaction be? What is the Gram reaction of non-acid-fast bacteria?

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Why isn’t the Gram stain used on acid-fast bacteria? If you did Gram stain acid-fast bacteria, what would their Gram reaction be? What is the Gram reaction of non-acid fast bacteria?
Why is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer.   Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.   The alcohol wash removes crystal violet and iodine from the bacteria.   Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.   Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
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