A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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1. Why is it important to use a small amount of bacteria when making a smear? Give at least 2 reasons. 2. What are the two reasons for “fixing” a smear? 3. Simple staining is useful for determining what three characteristics of a bacterial cell? 4. With respect to bacteria, define morphological arrangement. Explain thoroughly!

An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not used

Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:

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Question

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Question

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Question

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Answer 5

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Answer 6

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Answer 7

Chapter 3, Problem 8MCQ

Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2R Ch. 3 - Prob. 3R Ch. 3 - Prob. 4R Ch. 3 - Prob. 5R Ch. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7R Ch. 3 - Prob. 8R Ch. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Solution Summary: The author explains that a gram staining is based on the presence or absence of peptidoglycan in bacterial cells.

Concept explainers

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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Chapter 3 Solutions

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Solution Summary: The author explains that a gram staining is based on the presence or absence of peptidoglycan in bacterial cells.

Concept explainers

Introduction: A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

Want to see the full answer?

Chapter 3 Solutions

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).