Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 3, Problem 8MCQ
Summary Introduction

Introduction:

A gram staining is a differential stain most commonly used to classify bacteria in to gram-positive and gram-negative groups. It uses a primary stain (crystal violet), a decolorizer (alcohol/acetone) and a counter stain (safranin).

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2 2 1.5 1.0 0.67 5. (15 points) An individual comes into your clinic with a phenotype that resembles Down's syndrome. You perform CGH by labeling the patient's hobe DNA red and her mother's DNA green. Plot the expected results of the Red:Green ratio if: A. The cause of the syndrome was an inversion on one chromosome 21 in the child 0.5 1.5 1.0 0.67 0.5 21 p 12345678910 CEN q 123456789 10 11 12 13 14 15 16 17 18 19 B. The cause of the syndrome was a duplication of the material between 21q14 and 21q18 on one chromosome in the child 21 p 123456789 10 CEN q 12345678910 11 12 13 14 15 16 17 18 19 C. The mother carried a balanced translocation that segregated by adjacent segregation in meiosis I and resulted in a duplication in the child of the material distal to the translocation breakpoint at 21q14. 1.5 1.0 0.67 0.5 21 p 12345678910 CEN q 123456789 10 11 12 13 14 15 16 17 18 19 mom seal blo
4. You find that all four flower color genes map to the second chromosome, and perform complementation tests with deletions for each gene. You obtain the following results: (mutant a = blue, mutant b = white, mutant c = pink, mutant d = red) wolod Results of Complementation tests suld Jostum Mutant a b с Del (2.2 -2.6) blue white pink purple Del (2.3-2.8) blue white pink red Del (2.1 -2.5) blue purple pink purple Del (2.4-2.7) purple white pink red C d Indicate where each gene maps: a b ori ai indW (anioq 2) .8
lon 1. Below is a pedigree of a rare trait that is associated with a variable number repeat. PCR was performed on individuals using primers flanking the VNR, and results are shown on the agarose gel below the pedigree. I.1 1.2 II.1 II.2 II.3 II.4 II.5 II.6 11.7 III.1 III.2 III.3 III.4etum A. (5 points) What is the mode of inheritance? B. (10 points) Fill in the expected gel lanes for II.1, II.5, III.2, III.3 and III.4 C. (5 points) How might you explain the gel results for II.4?
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