Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope?     All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive.     All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive.     Gram negative cells will be clear, Gram positive cells will be purple.     Gram positive cells will be clear, Gram negative cells will be pink.     Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.

Out of twelve tests that can be performed:  Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test.  Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are:    Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis

Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it.  Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion

Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol. Which of the following outcomes would you expect? Both Gram-positive and Gram-negative bacteria would appear reddish-pink Both Gram-positive and Gram-negative bacteria would appear purple Gram-positive bacteria would appear purple and Gram-negative bacteria reddish-pink Gram-positive bacteria would appear reddish-pink and Gram-negative bacteria purple Gram-positive bacteria would appear colorless and Gram-negative bacteria reddish-pink

The GRAM stain method was applied on both pictures. What is the result found on each of them? A B 宁 键