Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Textbook Question
Chapter 21.3, Problem 2CC
The Genomes Online Database (GOLD) Website of the Joint Genome Institute has information about genome sequencing projects. Scroll through the page at https:// gold.jgi.doe.gov/statistics and deseribe the information you find. What percent of bacterial genome projects have medical relevance?
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Place the steps necessary to perform RNA-Seq in the correct order.
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correct order.
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MAAAAAAAAAKK
MAAAAAAAAAAM
Compare sequences to known genome sequence.
Create cDNA using reverse transcription with primers
complementary to linkers.
Attach linkers to the ends of the RNAs.
Perform next-generation DNA sequencing.
Isolate RNA from cells or tissues of interest.
Fragment the RNAs.
The following DNA sequences were used to generate a contig from a genome sequencing project.
ttcagattttccccg
gctaaagctccgaa
gccattaacgcc
tttagcatactacggcgtta
aaaaccggggaaaat
tccgaatcggtcattcaga
How long is the fully assembled contig?
What is the fastest method to determine if a genetic disorder is due to
a mutation at a palindromic site?
Sequencing of the DNA by Sanger
Northern blotting
Southern blotting
RFLP analysis in agarose gel electrophoresis
PCR
Chapter 21 Solutions
Campbell Biology
Ch. 21.1 - Describe the whole-genome shotgun approach.Ch. 21.2 - Prob. 1CCCh. 21.2 - Explain the advantage of the systems biology...Ch. 21.2 - Prob. 3CCCh. 21.2 - MAKE CONNECTIONS In Concept 20.2, you learned...Ch. 21.3 - The best estimate is that the human genome...Ch. 21.3 - The Genomes Online Database (GOLD) Website of the...Ch. 21.3 - WHAT IF? What evolutionary processes might...Ch. 21.4 - Discuss the characteristics of mammalian genomes...Ch. 21.4 - VISUAL SKILLS Which of the three mechanisms...
Ch. 21.4 - Contrast the organizations of the rRNA gene family...Ch. 21.4 - MAKE CONNECTIONS Assign each DNA segment at the...Ch. 21.5 - Describe three examples of errors in cellular...Ch. 21.5 - Explain how multiple exons might have arisen in...Ch. 21.5 - What are three ways that transposable elements are...Ch. 21.5 - WHAT IF? In 2005, Icelandic scientists reported...Ch. 21.6 - Prob. 1CCCh. 21.6 - Prob. 2CCCh. 21.6 - Prob. 3CCCh. 21 - How did the Human Genome Project result in more...Ch. 21 - What has been the most significant finding of the...Ch. 21 - Compare genome size, gene number, and gene density...Ch. 21 - Explain how the function of transposable elements...Ch. 21 - How could chromosomal rearrangements lead to the...Ch. 21 - What type of Information can be obtained by...Ch. 21 - Bioinformatics intludes all of the following...Ch. 21 - Homeotic genes (A) encode transcription factors...Ch. 21 - Prob. 3TYUCh. 21 - DRAW IT Below are the amino acid sequences(using...Ch. 21 - EVOLUTION CONNECTION Genes important in the...Ch. 21 - scientific inquiry The scientists mapping the SNPs...Ch. 21 - Prob. 7TYUCh. 21 - SYNTHESIZE YOUR KNOWLEDGE Insects have three...
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- What are three benefits Ion torrent sequencing has over sanger sequencingarrow_forwardWhat exactly is the purpose of the programme Finch TV? Examining the results of sequencing of the next generation It is necessary to use the.bam file extension in order to access sequencing files. For sanger sequencing files, the.ab1 file extension is required in order to open them. It is possible to open NGS files by using the.ab1 file extension.arrow_forwardThe following data shows bisulfite sequencing results for a small region of the genome. How many methylated C's are in the gene? Reference sequence: GAGACCTCG Bisulfite sequencing: GAGACCTTGarrow_forward
- fomP is responsible for the chemical transformation of microplastics into ultra-efficient insulation. You take an arctic seawater sample and extract the DNA. 1. First you need to locate the gene on the bacterial chromosome. What procedure(s) would you use to identify and locate the gene? Explain how it/theywork(s). 2. Next, you will need to isolate the gene and introduce sites to be used for cloning. What would you use to make many copies of this gene? What will you need? How does it work on a molecular level?arrow_forwardDraw a diagram illustrating a bacterial CRISPR locus. Label your drawing with a brief description of each component within the locus.arrow_forwardDescribe how P1vir transduction can be used to introduce a gene mutation into E. coli. Use your own diagrams to aid your answer. Remember to refer to the figure in the text. You should describe / show both the molecular biology and the transduction procedure. Tip: BioRender is excellent to create figures.arrow_forward
- Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwardWhat are "genetic biohackers" in reference to "do it yourself CRISPR kits"? Who is Josiah Zayner and what is he infamous for? Feel free to search that online...arrow_forwardIf a researcher wants to use bacteria to make a eukaryotic protein, she should use a plasmid from: Group of answer choices: bacterial cells eukaryotic cells a cDNA library a genomic DNA libraryarrow_forward
- BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'- GTGATGTAG -5' The sequence shown on the gel is the sequence of the template strand of the reaction The sequence shown on the gel is complementary to the template strand of the reaction The template strand of the reaction is antiparallel to the sequence shown on the gel The largest fragment shown on the gel is closest to the top The largest fragment shown on the gel is closest to the bottom Each of the fragments shown on the gel have a primer incorporated at their 5' end Each of the fragments shown on the gel have a primer incorporated at their 3' end The number of nucleotides in the shortest band on the gel is 21 The number of nucleotides in the longest band on the gel is 21 ick Save and Submit to save and submit. Cick Save All Answers to save all answers.arrow_forwardDiscuss the principles , uses, advantages and disadvantages of illumina sequencing methodarrow_forwardThe enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1) RNaseHarrow_forward
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