Becker's World of the Cell (9th Edition)
Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 20, Problem 20.6PS

(a)

Summary Introduction

To determine: Whether the statement, “An enhancer may increase the frequency of transcription initiation for its associated gene when it is located 1000 nucleotides upstream of the gene’s core promoter.” is true or false.

Introduction: A short part of the DNA which is involved in increasing the probability of transcription of a particular gene is called an enhancer. It is usually 50-1500 base pairs long. Usually, proteins such as activators bind it to the DNA.

(b)

Summary Introduction

To determine: Whether the statement, “An enhancer may increase the frequency of transcription initiation for its associated gene when it is in the gene’s coding region.” is true or false.

Introduction: A short part of the DNA which is involved in increasing the probability of transcription of a particular gene is called an enhancer. It is usually 50-1500 base pairs long. Usually, proteins such as activators bind it to the DNA.

(c)

Summary Introduction

To determine: Whether the statement, “An enhancer may increase the frequency of transcription initiation for its associated gene when no promoter is present” is true or false.

Introduction: A short part of the DNA which is involved in increasing the probability of transcription of a particular gene is called an enhancer. It is usually 50-1500 base pairs long. Usually, proteins such as activators bind it to the DNA.

(d)

Summary Introduction

To determine: Whether the statement, “An enhancer may increase the frequency of transcription initiation for its associated gene when it causes looping out of the intervening DNA.” is true or false.

Introduction: A short part of the DNA which is involved in increasing the probability of transcription of a particular gene is called an enhancer. It is usually 50-1500 base pairs long. Usually, proteins such as activators bind it to the DNA.

(e)

Summary Introduction

To determine: Whether the statement, “An enhancer may increase the frequency of transcription initiation for its associated gene when it causes alternative splicing of the DNA.” is true or false.

Introduction: A short part of the DNA which is involved in increasing the probability of transcription of a particular gene is called an enhancer. It is usually 50-1500 base pairs long. Usually, proteins such as activators bind it to the DNA.

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I am more confused. how about we start from begining, you post answers on here, and then we go from there?  1. Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source. 2. "Look carefully at the DNA sequence and identify the start site for transcription" 3. Click on the DNA sequence from the start site of transcription, select all of the sequence, and copy the sequence. Go to the National Center for Biotechnology Information (NCBI) website http://www.ncbi.nlm.nih.gov/. Click on BLAST on the right-hand side under “Popular Resources.” BLAST is a program that will allow you to find the protein sequence for the DNA sequence (gene) you submit. Next click on blastx (translated nucleotide protein). Paste the DNA sequence into the box under “Entry Query Sequence.” Scroll down and click BLAST. The search may take a few seconds; the page will keep updating until the search is completed. You do not need to enter any…
E32. In the technique of DNase I footprinting, the binding of a protein to a region of DNA protects that region from digestion by DNase I by blocking the ability of DNase I to gain access to the DNA. In the DNase I footprinting experiment shown here, a researcher began with a sample of cloned DNA 400 bp in length. This DNA contained a eukaryotic promoter for RNA polymerase II. The assembly of general transcription factors and RNA polymerase II at the core promoter is described in Chapter 12 (see Figure 12.14). For the sample loaded in lane 1, no proteins were added. For the sample loaded in lane 2, the 400-bp fragment was mixed with RNA polymerase II plus TFIID and TFIIB. 2 400 350 250 175 50 Which region of this 400-bp fragment of DNA is bound by RNA polymerase II and TFIID and TFIIB? || III ||| | ||||
Fill in the blanks.  In this schematic, [] represents a gene (DNA) that is transcribed and processed to form [], which are mRNA molecules.  [] represents one protein produced from the gene.  The molecule that will function as a transcription factor is [].
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