Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 19, Problem 2AL
You have recently established a pure culture of a new archaeon from soil. Describe the approaches you would use to identify your new microbe to the species level.
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You are working for the CDC studying outbreaks of foodborne infectious disease. Your latest case is
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O Each isolate is the same strain, of a different species
O Each isolate is a different strain, of different species
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The techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to:
Staining the unknown for initial characterization by microscopy.
Using a dichotomous key strategy to systematically rule out other organisms.
Testing the organism for key biochemical traits.
What is the importance of using a test/control organism in describing the cultural characteristics of an unknown bacterium?
Chapter 19 Solutions
Prescott's Microbiology
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - Prob. 2CCCh. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 3CCCh. 19.4 - Could a phylotype be considered an OTU? What about...
Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3CCCh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4CCCh. 19.6 - Prob. 1CCCh. 19.6 - Describe two different situations in which it...Ch. 19 - Prob. 1RCCh. 19 - Prob. 2RCCh. 19 - Prob. 3RCCh. 19 - Prob. 4RCCh. 19 - Prob. 5RCCh. 19 - Prob. 6RCCh. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 3ALCh. 19 - Prob. 4ALCh. 19 - Prob. 5ALCh. 19 - Prob. 6ALCh. 19 - Prob. 7AL
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- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?arrow_forwardThe process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaarrow_forwardIs it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What type of name should a microorganism have if it has been well identified but cannot yet be cultured in isolation?arrow_forward
- Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?arrow_forwardDefine the term sterile. What two methods did Pasteur used tomake solutions sterile?arrow_forwardWhat did pasteur prove when he showed that a cotton plug that had filtered air would trigger microbial growth when transferred to a sterile medium?what argument made previously was he addressingarrow_forward
- You are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 9) What makes the bacteria Gram negative?10) Approximately how long would it take you to finish the entire procedurearrow_forwardMedical Microbiology Give your own synthesis (using key words or phrases and presented using a flow chart or a schematic diagram) of the complexity and dynamics of microbial disease development.arrow_forwardHow does the streak plate technique help in isolating individual colonies of bacteria? Why streak multiple times on one plate? 3. While observing a plate inoculated with bacteria you observe a white-tan fuzzy growth. What can you interpret from this observation? Explain how this outcome could’ve occurred. 4. What are 3 possible errors that could take place as you culture your samples which could lead to contamination? State 3 and explain why. Microbiology classarrow_forward
- The use of culture-independent techniques has increased our understanding of microbial diversity without cultivation. Therefore, do microbiologists still need to attempt to grow new microbial species? Explain.arrow_forwardYou are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**arrow_forwardCompare and contrast three common laboratory techniques forseparating bacteria in a mixed sample.arrow_forward
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