Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 19, Problem 1AL

Consider the fact that the use of 16S rRNA sequencing as a taxonomic and phylogenetic tool has resulted in tripling the number of bacterial phyla. Why has the advent of this genetic technique expanded the currently accepted number of microbial phyla?

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The universal phylogenetic tree of life shows the divergence of Bacteria, Archaea, and Eukarya. Studies of Lokiarchaeota help to elucidate these relationships, but the first universal tree of life was constructed by Carl Woese using ribosomal RNA sequences. There are advantages to using different types of nucleic acid for different types of analyses. What is a specific advantage of using small subunit ribosomal RNA (SSU rRNA) gene sequences for phylogenetic analysis compared with DNA sequences? ▸ View Available Hint(s) SSU rRNA sequences accumulate mutations more rapidly than protein-encoding DNA. SSU rRNA is frequently transferred through horizontal gene transfer, whereas DNA sequences are not. Primers are required in DNA sequence analysis, but not for SSU rRNA sequence analysis. Although PCR products can be visualized with SSU rRNA, this is not the case with other forms of nucleic acids. DNA sequences are too highly conserved to work well for sequence analysis; individual species are…
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Prescott's Microbiology

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