Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?

Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 1CR

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Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2
Robert Koch developed a set of criteria (postulates) for conclusively demonstrating the aetiology (specific cause) of an infectious disease. Which of the following is not a postulate? The infectious agent must be isolated and cultured in vitro The disease is reproduced when a pure culture of the infectious agent is inoculated into a new susceptible host The infectious agent can be recovered from the experimentally-infected host The infectious agent is present in most cases of the disease
As a medical microbiologist, if you are presented with a patient suspected to have a bacterial disease, describe the various steps you would follow, to establish a laboratory diagnosis. Please keep brief - 8 sentences/dot points max.
Answer the following questions: 1. What was the first antibiotic and what was its importance? 2. What does resistance mean? 3. Who is affected by resistance? 4. What if the resistance problem is not solved? 5. Describe the structure of the bacterium (its parts) 6. Can bacteria change? explain 7. Why do Bacteria communicate, what is the purpose? 8. Explain how a bacterium achieves its resistance. 9. What is the use given to antibiotics in production animals? 10. Is this use in animals good practice? 11. Once resistance occurs, what has the scientific community had to do? 12. Do antibiotics only affect negative bacteria? explain. 13. What are the most feared diseases due to antibiotic resistance? 14. Should antibiotics be used against viruses? explain. 15. How can we avoid antibiotic resistance?
You have learned many different techniques so far this semester that allow us to identify pathogenic microbes in the clinical setting. Why do you think rapid methods are so helpful? Why are traditional tests such as metabolic tests not always used to ID clinically important microbes?
The following are the limitations of Koch's postulates EXCEPT: A. some pathogens cannot grow on artificial media and therefore cannot be identified as the causative agent of the disease B. some diseases involve multiple pathogens which produce similar symptoms making it difficult to pinpoint the causative agent C. some diseases are host-specific and re-inoculation may pose ethical concerns D. some microorganisms are present in the body fluids of the infected animal which make them difficult to be cultured
A patient is suffering from a bacterial pathogenic disease and you are given the patient's sample culture. As a clinical laboratory scientist, identify the 2 tests you would perform to identify the correct course of treatment. These tests have been taught in lab. Note: Identify the tests you would perform and NOT the disease or the specific drug to treat the disease.
You are working for the CDC studying outbreaks of foodborne infectious disease. Your latest case is an incidence of food poisoning in a fast food restaurant. 5 people have been hospitalized for food poisoning, so you are working with that hospital to identify the source of the outbreak. Hospital clinicians have taken samples from each of the 5 patients for culturing. Each bacterial culture grew one likely microbe, each labelled P1, P2, P3, P4, and P5. All 5 isolates appear to have the same colony morphology on the plates, and all appear to be Gram-negative rods 1 micron wide and 3 microns long under the microscope. In addition, all 5 isolates appear to perform the same on an API 20E test strip that tests bacteria for 20 biochemical abilities. No other testing has been done yet. How would you best describe your set of isolates at this moment? O Each isolate is the same strain, of a different species O Each isolate is a different strain, of different species O Each isolate is a different…
the following are the steps, listed in random order, for utilizing koch's postulates. what should be the second step out of the four listed? a. a microorganism is found to be present in every case of a disease. b. the same microbe discovered previously is isolated from the purposely infected host c. the pathogen is isolated and grown in pure culture d. the same disease results after isolated organism is inoculated into healthy host
Which of the following is NOT true about Koch's postulates? First developed by Robert Koch, the pioneering German microbiologist In the first step, the microbe that causes a naturally occurring disease is cultured from a "wild" (non-laboratory) animal which has that disease None of the other four answers (All are true about Koch's Postulates) They represent a process for showing a causal association between a specific microbe and a disease If the same microbe from a diseased "wild" (non-laboratory) host causes the same disease in a lab animal and it can be cultured from that lab animal, this proves that the microbe is the cause of the naturally occurring disease
Samples from an ill patient were collected by a physician for testing. A bacterial infection is suspected. Using the unknown flowcharts, a microbiologist conducted various tests to determine the identity of the unknown bacterial isolate. The resulting data is included in this presentation. Identify each test.