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Define the term sterile. What two methods did Pasteur used to
make solutions sterile?
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- What are Koch's postulates? Discuss the importance of these postulates in the identification of bacteriaa) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaWhy is it Koch's postulates cannot be used for the microbe E. Coli. What limitations does E.coli have?
- Use the following diagram to explain how Pasteur’s swan-necked flasks prevent contamination of sterile broth the flasks.Describe what happens to the sterile broth (a) after it has beenallowed to cool as in (b). What happens to the broth after theflask has been tipped enough to let the broth come in contactwith the dust and microorganisms and is tipped back as in (c)?you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)Is it likely that a pure culture could be isolated from the human body? Why or why not?
- what is means cross-contamination in animal cell culture? *Explain the different role, association and impact of Lactobacilli in food microbiology that you have studied. Use maximum 100 words.What did pasteur prove when he showed that a cotton plug that had filtered air would trigger microbial growth when transferred to a sterile medium?what argument made previously was he addressing
- What would happen if the enrichment method in the isolation of bacteriophage was omitted?the chloroform was not added to the enrichment?the 0.1 ml lysate - E.coli mix was plated directly on top of the bottom agar?Write a paragraph for when you once have Neisseria gonorrhoea bacteria in its pure form, what methods can you use to confirm the identity of this bacterium as N. gonorrhoeae? Mention what is the principle of those methods.Using the clinical question --"Does antibacterial foam decrease bacteria count on hands as much as hand washing with soap and water.” Question 1) Describe your study design and sample. 2) Will your findings be generalizable? Why or why not? 3) How does this exercise inform your interpretation of published research?.