The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes. Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger . This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

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Answer 1

Question

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

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Translation. Write the anti-codon sequence of the MRNA transcript. Translate the MRNA transcript into peptide sequence using both the 3 letter abbreviation and 1 letter abbreviation. ANTI-CODON 3' 5' SEQUENCE AMINO ACID N- C- SEQUENCE (3 letter terminus Abbreviation) Terminus AMINO ACID N- C- SEQUENCE (1 letter terminus Abbreviation) Terminus

AAAGAGAAAAGAAUA to AAAGAGAAAUGAAUA. Suppose the codon sequence has a single base pair mutation If the old protein sequence was Lys-Glu-Lys-Arg-Ile, what will be the new sequence encoded by the mutant gene? (Use the 3-letter amino acid abbreviations with hyphens and no spaces in between, i.e. Ser-Asn-Tyr-Leu-Pro.) Submit Answer Retry Entire Group No more group attempts remain

An extra piece. In one type of mutation leading to a form of thalassemia, the mutation of a single base (G to A) generates a new 3' 3' splice site (blue in the illustration below) akin to the normal one (yellow) but farther upstream. Normal 3' end of intron 5' CCTATTGGTCTATTITCCACCCITAGGCTGCTG 3' 5' CCTATTAGTCTAIIIICCACCCTTAGGCTGCTG 3' What is the amino acid sequence of the extra segment of protein synthesized in a thalassemic patient having a mutation leading to aberrant splicing? The reading frame after the splice site begins with TCT.

Answer 2

Question

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

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Answer 3

Question

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

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Answer 4

Question

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Expert Solution & Answer

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Answer 5

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 6

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 7

Chapter 19, Problem 19.6PS

(a)

Summary Introduction

To explain: The result that when puromycin is added to a cell-free system containing all the necessary machinery for protein synthesis, incomplete polypeptide chains are released from the ribosomes.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 8

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 9

(b)

Summary Introduction

To determine: The polypeptide chain end to which the puromycin will be attached.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 10

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 11

(c)

Summary Introduction

To determine: The puromycin will bind to A or P site on the ribosome or to both..

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 12

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 13

(d)

Summary Introduction

To explain: If puromycin is a better inhibitor of protein synthesis in eukaryotes or bacteria.

Introduction: Puromycin is an antibiotic obtained from Streptomyces alboniger. This is primarily involved in the inhibition of protein synthesis. It is an inhibitor of translation in both the cells of prokaryotic and eukaryotic organisms.

Answer 14

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Answer 16

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Answer 17

Ch. 19 - Suppose a tRNA has the anticodon 3-CGU-5. What two...Ch. 19 - Kanamycin is an antibiotic that binds to bacterial...Ch. 19 - You are working in a lab to identify new alleles...Ch. 19 - Suppose you are using GFP fusion proteins to study...Ch. 19 - Enzymes can undergo allosteric regulation or...Ch. 19 - Suppose you use molecular biology techniques to...Ch. 19 - Prob. 19.1PS Ch. 19 - Tracking a Series of Mutations. The following...Ch. 19 - Prob. 19.3PS Ch. 19 - Prob. 19.4PS

Solution Summary: The author explains that puromycin is an antibiotic obtained from Streptomyces alboniger, which is primarily involved in the inhibition of protein synthesis.

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