Chapter 18.3, Problem 2R

Summary Introduction

To review:

The process of reverse transcriptase-polymerase chain reaction (RT-PCR), which can be used to compare the mRNA’s (messenger ribonucleic acid) made by muscle versus liver tissues.

Introduction:

Polymerase chain reaction (PCR) is a technique used to amplify the sequence of a genetic material. When an enzyme called reverse transcriptase is used in PCR procedures so as to create and amplify a specific complementary deoxyribonucleic acid (cDNA) sequence, then this technique is termed as RT-PCR. It has, nowadays, become an invaluable tool for studying the specific gene expressions in cells, tissues, and organisms. The oligonucleotide primers are used to initiate the amplification of the desired deoxyribonucleic acid (DNA) sequence after it gets attached to either one or both the ends of the DNA template.