Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 18.1, Problem 2TQ
Summary Introduction
To review:
The group of bacteria that are stained either by Gram-positive stain or by the Gram-negative stain and the group of bacteria that are stained by both. Also, mention the group for which the
Introduction:
Gram-positive bacteria take up the crystal violet stain. When viewed through an optical microscope, they appear to be purple in color. The thick peptidoglycan layer in the bacterial cell wall after being washed away still retains the stain. The Gram-negative bacteria are unable to retain the violet stain. The peptidoglycan layer in Gram-negative species is much thicker.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What is the shape of bacteria under a microscope, what color is there,
and is there Gram staining is positive or negative.
How do gram-positive and gram-negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties?
In lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative
bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the
counter-stain safranin).
a) What is the biological molecule that is present in these bacteria that produces different results
between Gram-positive and Gram-negative bacteria (be specific!) AND
b) What is the difference between Gram-positive and Gram-negative bacteria that creates the
different staining patterns (purple vs. pink)?
(2-3 sentences max)
Chapter 18 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria responsible in any way for the Gram stain reaction?arrow_forwardAll of the following are correctly matched and can be found in bacteria EXCEPT: None of the other four answers (all are correctly matched and can be found in bacteria) Nucleus membrane-bound organelle containing multiple chromosomes Ribosomes - site of protein synthesis Cell wall contains peptidoglycan Cell (plasma) membrane - encloses cytoplasm; phospholipid bilayer with proteinsarrow_forward16:53 In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol The counterstain (safranin) causes the purple color to develop None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The primary stain, crystal violet, is dark purple O LTE O O ×arrow_forward
- What is meant by Gram positive? Explain the mechanism of Gram positive staining.arrow_forwardWhat shape is the gram negative and gram positive?arrow_forwardIdentify the cellular morphology for this Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? What is the staining dye? [Choose ] [Choose ] Cocci Singles no arrangement Pleomorphic Chains Gram-negative Crystal Violet Phenol Red Bacilli Gram-positive Clusters Safraninarrow_forward
- What is the name of bacteria for each gram staining and identify the shape and the arrangement?arrow_forwardIn the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purplearrow_forwardWould this bacteria be described as gram negative or gram positive? Describe the shape and arrangement of the bacteria.arrow_forward
- For each of the following stains, list each of the chemicals used (in order), briefly describe what you would observe under the microscope, and list examples for each. - Gram stain (list color and example for Gram + and Gram -) - Acid Fast (list color and example for Acid Fast + and -) - Negative Stain (list color of organism stained with negative stain) - Capsule Stain (list color and example of organism with capsule) - Spore stain (list color and example of spore and cell)arrow_forwardIn the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The counterstain (safranin) causes the purple color to develop The primary stain, crystal violet, is dark purple Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcoholarrow_forwardWhy do low concentrations of alcohol inhibit Gram-negative bacteria more than Gram-positive bacteria? Explain how the alcohol interacts with: 1) Gram-negative cell wall structures 2) Gram-positive cell wall structuresarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Bacterial Structure and Functions; Author: Osmosis;https://www.youtube.com/watch?v=b15Hy3jCPDs;License: Standard youtube license