Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 18, Problem 4PDQ
Summary Introduction
To review:
Comparison of whole genome shot-gun sequencing to map based cloning approach.
Introduction:
Whole genome sequencing was developed as an alternative to cloning approach. It requires splitting of deoxyribonucleic acids (DNA) fragments in short overlapping fragments, which are then computationally aligned and sequence of a chromosome can be read.
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Chapter 18 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 18 -
CASE STUDY | Your microbiome may be a risk factor...Ch. 18 - CASE STUDY|Your microbiome may be a riskfactor for...Ch. 18 -
CASE STUDY | Your microbiome may be a risk...Ch. 18 -
HOW DO WE KNOW?
1. In this chapter, we focused on...Ch. 18 - Review the Chapter Concepts list on page 345. All...Ch. 18 - Prob. 3PDQCh. 18 - Prob. 4PDQCh. 18 - Prob. 5PDQCh. 18 - Prob. 6PDQCh. 18 - Prob. 7PDQ
Ch. 18 -
8. BLAST searches and related applications are...Ch. 18 - Describe the human genome in terms of genome size,...Ch. 18 - Prob. 10PDQCh. 18 -
11. Annotation involves identifying genes and...Ch. 18 - Through the Human Genome Project (HGP), a...Ch. 18 -
13. Describe the significance of the Genome 10K...Ch. 18 - Prob. 14PDQCh. 18 - Prob. 15PDQCh. 18 - Prob. 16PDQCh. 18 -
17. Annotation of the human genome sequence...Ch. 18 - Prob. 18PDQCh. 18 - Prob. 19PDQCh. 18 - Prob. 20PDQ
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- What is the difference between whole exome and whole genome sequencing?arrow_forwardWhat are the main differences between whole genome sequencing and whole exome sequencing?arrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forward
- Consider the diagram below. What Boolean phrase would you use to look for articles related to the ethics of cloning in organisms other than human beings? with explanation pleasearrow_forwardDiscuss the advantages of next-generation sequencing technologies.arrow_forwardCompare the possible differences between a eukaryotic protein-encoding gene cloned by PCR and the same gene cloned by reverse transcriptase PCR (RT–PCR).arrow_forward
- Place the steps of whole genome sequencing in order.arrow_forwardCompare and contrast the use of PCR and gene cloning for amplifying DNA fragments. What are the advantages and disadvantages of each method?arrow_forwardDescribe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.arrow_forward
- What is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.arrow_forwardHow does cloning be helpful? Cite specific field or areas where cloning is beneficial. Explain in 10 sentences, please.arrow_forwardQ7) What are things that are common in cloning vectors? Why are they useful?Q8) Using the pCR 2.1 vector, what antibiotics would a cell with the vector be resistant to?Q9) What does B-galactosidase do?arrow_forward
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