Discuss the advantages of next-generation sequencing technologies.

Q: Discuss three important advances that have resulted from gene cloning.

A: Gene cloning or DNA cloning is a technique of molecular biology that is used to produces many…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: List the three features of a cloning vector and why these elements are needed

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: Describe the methods of site-directed mutagenesis and CRISPR-Cas technology.

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Determine how screening is carried out if pCR®II-TOPO® is used for cloning?

A: There are many applications of PCR as used in DNA fingerprinting to identify an individual from…

Q: Outline and discuss the steps in DNA cloning

A: Gene cloning, also known as molecular cloning, is the method of isolating a DNA sequence of interest…

Q: Discuss how gene databases and genomic methods are used to study human genetic diseases.

A: The most powerful and versatile approach to looking out genomic databases.

Q: Exaplain the two methods to study the contents of transcriptomes which are microarray analysis and…

A: Transcriptome analysis may be used to find novel genes and isoforms that are not already cataloged…

Q: What is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.

A: Answer-  Vector- vector is a DNA molecule that is used to carry a foreign DNA into the host cell. It…

Q: Explain the development of next generation sequencing (NGS)technology ?

A: The words next-generation sequencing (NGS), massively parallel sequencing (MPS), and deep…

Q: Briefly describe the creation of a transgenic mouse in your lab.

A: A transgene is a gene that has been transferred from one species to another, either spontaneously or…

Q: Briefly outline how a mutagenesis screen is carried out.

A: Mutagenesis screening can be defined as an experimental technique that helps to screen out those…

Q: Describe the principles behind recombinant DNA technology.

A: Introduction Gene cloning is the technique to produce the exact copies of desire gene by utilizing…

Q: Explain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait…

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?

A: Early efforts at sequencing genes ere troublesome, when Gilbert and Maxam reported the sequence of…

Q: What would be your experimental strategy and the lab materials required to clone the complementary…

A: Aim - To clone the complementary human gene using a mammalian gene in conjunction with PCR so, first…

Q: Give three important characteristics of cloning vectors.

A: Cloning is an important process in which bacterial cells containing recombinant DNA can be readily…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: Discuss the evolution of sequencing technologies. What are some of the advantages of the new…

A: DNA sequencing DNA sequencing involves techniques and methodologies by which the order of the…

Q: Explain why a selectable marker is frequently included in a cloning vector.

A: A cloning vector is a short bit of DNA that may be kept in an organism for a long time and into…

Q: Explain two parameters that should be considered when designing primers for PCR.

A: PCR stands for Polymerase Chain Reaction. Using this technique, a fragment of DNA can be amplified.…

Q: Briefly describe what CRISPR technology does

A: Gene is a particular segment of deoxyribose nucleic acid (DNA) that is responsible for the…

Q: Explain how DNA technology can be used to treat geneticdiseases.

A: Genetic disease refers to illness that result due to defects in the gene, leading to malfunction or…

Q: Describe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular…

A:

Q: Define molecular cloning

A: The DNA molecules that are formed by laboratory methods involving genetic recombination is called…

Q: What are other potential applications of syntheticgenomes and synthetic biology?

A: Synthetic genomics and synthetic biologies are artificial biological products and modified…

Q: Define mutagen and describe how mutagens are usedin genetic research.

A: Mutation is defined as a change or alteration that occurs in a DNA of a cell. It occurs when there…

Q: Briefly present experimental and practical benefits of using PCR in DNA cloning process.  Give some…

A: The DNA Cloning is the production of a large number of identical DNA molecules from a single…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.