Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 17, Problem 17.10PS
Mutatis mutandis You work in a lab that uses the nematode C. elegans as a model organism. You find that one of the strains you are using has an inexplicably high incidence of mutations. It turns out this is because this strain is carrying an active transposon that was accidentally introduced into the strain. Indeed, such strains are called “mutator” strains by labs that work on worms. Why would a transposon cause such a high incidence of mutation?
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The genetic alteration responsible for sickle-cell anemia in humans involves:
a transition mutation from A to G, substituting glutamic acid for valine in a-globin
a transversion mutation from T to A, substituting valine for glutamic acid in b-globin
a transition mutation from T to C, substituting valine for glutamic acid in b-globin
a transversion mutation from G to C, substituting glutamic acid for valine in a-globin
a frameshift mutation of one ATC codon, removing glutamic acid from b-globin
Most organisms display a circadian rhythm, a cycling of biological processes that is roughly synchronized with day length. In Drosophila, pupae eclose (emerge as adults after metamorphosis) at dawn.
a)Using this knowledge how would screen for Drosophila mutants that have an impaired circadian rhythm?
b)In each case, how would you clone the genes you identified by mutation?
Chapter 17 Solutions
Becker's World of the Cell (9th Edition)
Ch. 17 - The theoretical amplification accomplished by n...Ch. 17 - Bacterial replication and that in typical...Ch. 17 - Nonhomologous end-joining and synthesis-dependent...Ch. 17 - Prob. 17.3CCCh. 17 - Meselson and Stahl Revisited. For each of the...Ch. 17 - DNA Replication. Sketch a replication fork of...Ch. 17 - More DNA Replication. The following are...Ch. 17 - QUANTITATIVE Still More DNA Replication. Suppose...Ch. 17 - The Minimal Chromosome. To enable it to be...Ch. 17 - Prob. 17.6PS
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