Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 16, Problem 14P
Summary Introduction

To analyze:

Access to http://blast.ncbi.nlm.nih.gov/Blast.cgi and type in the sequence below in the link nucleotide blast, it is broken into codons to make it simple to copy.

5' ATG TTC GTC AAT CAG CAC CTT TGT GGTTCT CAC CTC GTT GAA GCTTTG TAC CTT GTTTGC GGT GAA CGT GGT TTC TTC TAC ACT                       CCT AAG ACT TAA 3'

Collect the information on the basis of steps mentioned in the question and determine the DNA sequence.

Introduction:

In bioinformatics, BLAST stands for “basic local alignment search tool” that finds the region of local similarity between the biological sequences. This algorithm compares a DNA sequence or an amino acid sequence to the sequences that are in the databases and calculates the statistical significance of the sequence matches. BLAST can be used to identify the members that belong to one gene family. There are different types of BLAST present used according to the query sequence.

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Based on the following wild type DNA sequence, indicate if each of the mutations should be classified as : insertion, deletion, missense, nonsense, silent (Use the provided Genetic Code table and remember you have been given DNA sequence).    Wild Type:      5’ ATG GCT AGA GTC GAG TTG 3’ Mutant 1:         5’ ATG GCA GAG TCG AGT TG 3’ Mutant 2:         5’ ATG GCT TGA GTC GAG TTG 3’ Mutant 3:         5’ ATG GCT AGA GTT GAG TTG 3’ Mutant 4:         5’ ATG GCT AGA AGT CGA GTT G 3’ Mutant 5:         5’ ATG GCT AGA ATC GAG GTT 3’
The DNA sequence below is transcribed from left to right (the partner/coding strand is shown). Using this sequence, write the sequence of the polypeptide that results from this gene. Be sure to appropriately label the ends of the molecule. 5'-ATGCACGGCGACTAG-3' Second letter A UAU Tyr UAC First letter U A G U UUU1 UUC UUA LOU Leu CUU CUC CUA CUG Phe GUU GUC GUA GUG Leu AUU AUC lle AUA AUG Met Val C UCU UCC UCA UCG CCU CCC CCA CCG ACU ACC ACA ACG GCU GCC GCA GCG Ser Pro Thr Ala CAU His CAC CAA CAG Gin AAU Asn AAC AAA 1 Lys AAG LYS G {}a UAA Stop UGA Stop A UAG Stop UGG Trp GAU 1 GAC Asp GAA GIU Glu GAGJ UGU UGC CGU CGC CGA CGG AGU AGC AGA AGG Cys GGU GGC GGA GGG Arg Ser Arg DOA DOA DOA DUTO Third letter Gly
A protein uses a gene template in 3' to 5' direction. What are the amino acid sequences following this DNA chain? 5'-ATGTCGACAGCCTAA-3' First Second Letter Third Letter Letter phenylalanine phenylalanine serine tyrosine tyrosine cysteine cysteine U serine leucine serine stop stop A leucine serine stop tryptophan arginine G leucine proline histidine leucine proline histidine arginine leucine proline glutamine arginine arginine A leucine proline glutamine isoleucine threonine asparagine asparagine lysine serine isoleucine threonine serine A isoleucine threonine arginine methionine threonine lysine aspartate arginine valine alanine glycine glycine valine alanine aspartate C G valine alanine glutamate glycine glycine A valine alanine glutamate O Met-lle-Thr-Ala-STOP O Met-Ser-Trp-Arg-STOP O Met-Tyr-Thr-Arg-STOP O Met-Ser-Thr-Ala-STOP

Chapter 16 Solutions

Genetic Analysis: An Integrated Approach (3rd Edition)

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