Determine the approach by which two-hybrid method facilitate the discovery of protein- protein interactions. Explain the reason for the false-positive results (where the proteins encoded by two clones interact in the two-hybrid system but do not interact in the organism in which they naturally exist) obtained sometimes. Also the reason for the occurrence of false – negative (where the two proteins interact in vivo but fail to interact in the two-hybrid system) results. Introduction: There are many high-throughput technologies developed for proteomic analyses, including techniques to study protein expression, modification, and protein-protein interactions. The study of protein- protein interactions show whether and how different proteins interact, and provide information on the functioning of biological systems by identifying sets of proteins that form complexes. Two hybrid methods are successfully applied to many model systems, providing information on their protein interaction networks.

Question

Want to see the full answer?

Answer 1

Question

Chapter 16, Problem 13P

Summary Introduction

To analyze:

Determine the approach by which two-hybrid method facilitate the discovery of protein- protein interactions. Explain the reason for the false-positive results (where the proteins encoded by two clones interact in the two-hybrid system but do not interact in the organism in which they naturally exist) obtained sometimes. Also the reason for the occurrence of false – negative (where the two proteins interact in vivo but fail to interact in the two-hybrid system) results.

Introduction:

There are many high-throughput technologies developed for proteomic analyses, including techniques to study protein expression, modification, and protein-protein interactions. The study of protein- protein interactions show whether and how different proteins interact, and provide information on the functioning of biological systems by identifying sets of proteins that form complexes. Two hybrid methods are successfully applied to many model systems, providing information on their protein interaction networks.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Describe the following techniques for determining gene or protein function with advantages disadvantages and limitations. i)forward genetics(random mutagenesis) ii)reverse genetics(altering the function of particular gene) iii)genetic analysis(double or higher order mutant combination) iv) expression studies RNA and protein v)protein interaction studies(Y2H and other technique)

We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…

In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f, g, h and i pairs of genes were analyzed for co-transformation with the following results: Gene Pair Co-transformation g+ i+ f+ i+ yes no f+ h+ Уes f+ g+ no g+ h+ h+ i+ no уes What is the linear order of these genes relative to cach other?