Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 16, Problem 19P
Symbiodinium minutum is a dinoflagellate with a genome size that encodes more than
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Chapter 16 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 16 - You have discovered a new species of Archaea from...Ch. 16 - 16.2 Repetitive DNA poses problems for genome...Ch. 16 - 16.3 When the whole-genome shotgun sequence of the...Ch. 16 - How do cDNA sequences facilitate gene annotation?...Ch. 16 - 16.5 How do comparisons between genomes of related...Ch. 16 - 16.6 You are designing algorithms for the...Ch. 16 - 16.7 You have sequenced a region of the Bacillus...Ch. 16 - You have just obtained 100-kb of genomic sequence...Ch. 16 - 16.9 The human genome contains a large number of...Ch. 16 - Based on the tree of life in Figure 16.12, would...
Ch. 16 - 16.11 When comparing genes from two sequenced...Ch. 16 - 16.12 What is a reference genome? How can it be...Ch. 16 - Prob. 13PCh. 16 - Prob. 14PCh. 16 - 16.16 Consider the phylogenetic tree below with...Ch. 16 - You have isolated a gene that is important for the...Ch. 16 - 16.18 When the human genome is examined, the...Ch. 16 - Symbiodinium minutum is a dinoflagellate with a...Ch. 16 - Substantial fractions of the genomes of many...Ch. 16 - 16.21 A modification of the system, called the ...Ch. 16 - 16.22 A substantial fraction of almost every...Ch. 16 - 16.23 In the globin gene family shown in Figure ,...Ch. 16 - You are studying similarities and differences in...Ch. 16 - In conducting the study described in Problem 24,...Ch. 16 - Prob. 26PCh. 16 - Prob. 27PCh. 16 - Prob. 28PCh. 16 - Prob. 29P
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- In a hypothetical scenario, you wake up one morning to your roommate exclaiming about her sudden hair growth. She has been supplementing her diet with a strange new fungus purchased at the local farmer’s market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the harE protein) that stimulates hair growth. You construct a fungal genomic DNA library in E. Coli with the hope of cloning the harE gene. If you succeed you will be a billionaire! You obtain DNA from the fungus, digest it with a restriction enzyme, and clone it into a vector. b) You clone your digested genomic DNA into this vector. The E. coli (bacteria) cells that you will transform to create your library will have what phenotype prior to transformation?c) How do you distinguish bacterial cells that carry a vector from those that do not?d) How do you distinguish bacterial cells that carry a recombinant vector from those that carry the original cloning vector?arrow_forwardAgrobacterium tumefaciens is a bacterium that infects a wide range of broad-leaved plants. During infection, a plasmid called Ti is transferred into the plant cells, where that plasmid DNA is integrated into the plant’s genome. Some of the genes encoded by this plasmid are expressed by host cell machinery in the plant cell. These gene products produce plant hormones which stimulate cell division in the infected cells, causing the development of a plant tumor which provides an environment for that bacterium to grow. You are studying a novel isolate of A. tumefaciens and want to determine which of the genes on the Ti plasmid are expressed in the bacterium, and which are expressed in the plant. You start by sequencing the Ti plasmid. a) Considering the DNA sequence of Ti, describe two specific genetic features that would help you to differentiate whether a given gene is likely expressed in the plant or bacterium. b) You collect plant tumor tissue, containing both bacterial and plant…arrow_forwardIn your own words, discuss the life cycle of E. histolytica.arrow_forward
- The phylogenetic tree of the OCTN homologs below was generated with the following accession numbers as basis: NP_957143.1 - OCTN of unknown function CDM63451.1 - OCTN specific for ergothioneine transport XP_021324939.1 - OCTN specific for carnitine transport Design the phylogenetic tree to make it look more interesting by highlighting the distinct clades or putting a visual emphasis on the gene you are trying to analyze. Provide a caption for this figure.arrow_forwardThree haploid fungal mutants that require compound W for growth were isolated. Each mutant contains a recessive allele in a single gene. Three compounds (A, B and C) in the biosynthetic pathway to W are known, but their order in the pathway is unknown. Each compound is tested for its ability to support the growth of each of the three mutants. Phenotypes of all of the three mutants are shown in the following table (“+" indicates growth, "-" indicates no growth). A C W Mutant 1 Mutant 2 Mutant 3 What would be the phenotype of a haploid mutant that contains both mutant alleles in mutant 2 and 3? Phenotype refers to growth or absence of growth on compounds A, B, C and WN. O Like mutant 1 O Like mutant 2 Like mutant 3 O Like wild typearrow_forwardA hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forward
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License