Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 16P
Suppose you are examining a newly found plant species, and you want to determine whether the inheritance of mtDNA is maternal, paternal, or biparental. You find that in the population two variants of mtDNA exist that can be distinguished by size differences of PCR amplification products made with a particular pair of primers. You first perform PCR analysis on DNA isolated from a leaf on each of two individual plants. Then you cross eggs from plant 1 with pollen from plant 2, obtain four seedlings, and perform PCR analysis with DNA from each of the whole seedlings. The results are shown below.
| a. | Assuming plant 2 is homoplasmic, do these results exclude any of the three possible models for the inheritance of mtDNA in this species (maternal, paternal, or biparental)? |
| b. | What experiment could you perform to distinguish between the models that remain? |
| c. | What experiment(s) could you perform to check your assumption that plant 2 is in fact homoplasmic? Why is such an experiment necessary to make a conclusion about the mode of inheritance of mtDNA in this species? |
| d. | Explain the differing proportions of the two forms of mtDNA in the four seedlings. Be as specific as possible. |
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Consider the first category of test-cross offspring shown in figure 8.2 (+b, LS). Consider also that the parents of the heterozygous female flies in the test cross had the following genotypes: bb, SS, and +, LL.
A. What would be the physical phenotype of these flies?
B. If PCR was conducted with the DNA of one of these flies using the primers for the molecular marker, what would be the appearance of the bands on an electrophoresis gel with the PCR products?
C. If the gene for black body and the locus for the molecular marker (L long or S short) were unlinked, what proportion of the test-cross progeny would be black flies that are heterozygous for the molecular marker? What proportion would be flies with normal body color, which are homozygous for one form of the molecular marker?
D. If the gene for black body and the locus for the molecular marker were linked, how would the proportion of flies be different?
A region of the genome is amplified by PCR to analyze two closely linked SNPs. PCR products from individual A were labelled with a red-fluorescing dye and those from individual B labelled with a green-fluorescing dye. These PCR products were mixed and hybridized to an oligonucleotide microarray as shown in the figure below. What can we conclude about the data shown?
Select all true:
a. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M4 and M5
b. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M3 and M6
c. Sanger sequencing traces of these PCR products would show the same double peaks at the same positions for these two individuals
d. analyzing this PCR amplified region with conventional Sanger sequencing would be more accurate than the microarray analysis
e. individuals A and B could be siblings who share a parent that is homozygous for one of the haplotypes
{urgent}
ou are studying a new gene “X” that you think controls skin color in Bearded Dragons. In order to determine what gene X does, you need lots of gene X DNA to work with. So, you decide to amplify it through the process known as PCR. You have a forward and a reverse primer that have been designed to gene Xf – a gene in frogs that is similar to the one found in the Bearded Dragon. You are pretty sure that these two primers will work in Bearded Dragons, so you decide to go ahead with the PCR. In frogs, the Forward Xf primer binds gene Xf between nucleotides 32 and 47. The Reverse Xf primer binds between nucleotides 1110 and 1135.
a. What size PCR product would these two primers create if they amplified the frog gene Xf??
Chapter 15 Solutions
Genetics: From Genes to Genomes
Ch. 15 - Match each numbered item with the most closely...Ch. 15 - Assuming human cells have on average 1000...Ch. 15 - Reverse translation is a term given to the process...Ch. 15 - The human nuclear genome encodes tRNAs with 32...Ch. 15 - The human mitochondrial genome includes no genes...Ch. 15 - How do you know if the halibut you purchased at...Ch. 15 - Is each of these statements true of chloroplast or...Ch. 15 - Suppose you are characterizing the DNA of a...Ch. 15 - An example of a gene-targeting DNA plasmid vector...Ch. 15 - Which of the following characteristics of...
Ch. 15 - The Saccharomyces cerevisiae nuclear gene ARG8...Ch. 15 - The so-called hypervariable regions HV1 and HV2 of...Ch. 15 - Suppose a new mutation arises in a mitochondrial...Ch. 15 - Describe at least two ways in which the...Ch. 15 - Why are severe mitochondrial or chloroplast gene...Ch. 15 - Suppose you are examining a newly found plant...Ch. 15 - A form of male sterility in corn is inherited...Ch. 15 - Plant breeders have long appreciated the...Ch. 15 - A mutant haploid strain of Saccharomyces...Ch. 15 - Prob. 20PCh. 15 - What characteristics in a human pedigree suggest a...Ch. 15 - The first person in the family represented by the...Ch. 15 - In 1988, neurologists in Australia reported the...Ch. 15 - If you were a genetic counselor and had a patient...Ch. 15 - Kearns-Sayre syndrome KSS, Pearson syndrome, and...Ch. 15 - Many clinically relevant mitochondrial diseases...Ch. 15 - Leigh syndrome is characterized by psychomotor...Ch. 15 - All mutations in mitochondrial genes ultimately...Ch. 15 - How could researchers have determined that the...
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