Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 20P
Summary Introduction

a.

To determine:

The phenotypes of grande and petite yeast cells with respect to the extraction of cell energy from the environment.

Introduction:

The bread mold or the Saccharomyces cerevisiae is extensively studied in the field of genetics. The fusion of the gametes gives rise to the spores that are in a specific structure called ascus. The spores formed are eight in number and follow Mendelian genetics. The four spores contain one allele while the other four spores contain the second allele.

Summary Introduction

b.

To determine:

The difference between the uniparental and biparental inheritance as seen in the bread mold and the validation of the paradoxical statement.

Introduction:

Microorganisms are small microscopic organisms that are present almost everywhere. They inhabit nearly all the areas from the air, water, soil, hot springs, snow, and so on. The microbes can be infectious or non-infectious. Microorganisms can be unicellular (single-celled) or multicellular.

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You have identified five genes in S. cerevisiae that are induced when the yeast are grown in a high-salt (NaCl) medium. To study the potential roles of these genes in acclimation to the growth in high-salt conditions, you wish to examine the phenotypes of loss- and gain-of-function alleles of each. How will you do this?
Schizosaccharomyces pombe, also known as "fission yeast," is a powerful model organism in molecular and cell biology. While performing a genetic screen, you discover an auxotrophic S. pombe strain that is unable to synthesize one or more vitamins. The following table represents the key experiments you performed during your genetic screen. Fill in the table with the outcome of each experiment for your mutant strain (using + for growth and - for no growth). Medium Rich media Minimal media Minimal media + all vitamins Minimal media + all amino acids Growth Wild-type + + + + Mutant + + + > > >
Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction). Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium alone, however, they can grow on medium supplemented with adenine. All three yeast strains are homozygous for the underlying alleles. When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium alone and require adenine supplementation; when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium alone and do not require adenine. After crossing the F1 generation of the cross between mutant strains 1 and 3, you count and determine the phenotypes of 1,000 colonies (here a colony is equivalent to an individual):  563 colonies that can grow on minimal medium alone; 437 colonies that require adenine…

Chapter 15 Solutions

Genetics: From Genes to Genomes

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