Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 15, Problem 19P
A mutant haploid strain of Saccharomyces cerevisiae (yeast) called cox2-1 was found that was unable to grow on media containing glycerol as the sole source of carbon and energy. (Glycerol is a nonfermentable substrate for yeast.) This strain could, however, grow on the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial protein called cytochrome c oxidase.
| a. | Explain why cox2-1 cells can grow on medium containing glucose but not on glycerol medium. |
| b. | When cox2-1 was crossed with a wild-type yeast strain and the resultant diploid cells were allowed to grow mitotically, it was found that about half the diploid clones were able to grow on glycerol, while the other half could not. The diploid clones that could grow on glycerol were induced to sporulate, and they yielded tetrads with four spores that were all able to grow on glycerol medium. In all of these tetrads, two of the haploid progeny were of mating type a and two of mating type α. The diploids that could not grow on glycerol could not sporulate. What do the results of the mating say about the location of the cox2-1 mutation? |
| c. | A different mutant strain of yeast called pet111-1 is also unable to grow on glycerol medium but still can grow on glucose medium. These mutant cells similarly lacked the cytochrome c oxidase. When pet111-1 was crossed with a wild-type haploid strain of the opposite mating type, the resultant diploids were able to grow on glycerol and yielded asci that all showed a 2:2 segregation of haploid cells that could or could not grow on glycerol. Explain these results in light of your answer to part (b) |
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A mutant haploid strain of Saccharomyces cerevisiae(yeast) called cox2-1 was found that was unable togrow on media containing glycerol as the sole sourceof carbon and energy. (Glycerol is a nonfermentablesubstrate for yeast.) This strain could, however, growon the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial proteincalled cytochrome c oxidase.a. Explain why cox2-1 cells can grow on mediumcontaining glucose but not on glycerol medium.b. When cox2-1 was crossed with a wild-type yeaststrain and the resultant diploid cells were allowedto grow mitotically, it was found that about half thediploid clones were able to grow on glycerol, whilethe other half could not. The diploid clones thatcould grow on glycerol were induced to sporulate,and they yielded tetrads with four spores that wereall able to grow on glycerol medium. In all of thesetetrads, two of the haploid progeny were of matingtype a and two of mating type α. The diploids thatcould not…
In early studies of cultured cells, a respi-
ration-deficient mutant of Chinese hamster
fibroblasts was found. The respiration rate
of the mutant cell line was only 8% of the
wild type cell line. It was determined that
that all enzymes of the Krebs cycle or Tri-
carboxylic Acid Cycle were present in the
mutant. Also, absorption spectra of the cy-
tochrome content of mitochondria showed
no deficit in cytochrome content. Various
assays were carried out and results are il-
lustrated below.
moles 02 consumed / hr/mg protein
2.0)
15
0.5
A
Succinate a-glycerol-
phosphate
0.4
0.3
0.2
01
4.
B
Malate Glutamate d-Keto- Isocitrate
glutarate
(a)
Rates of reduction of O2 catalyzed by wild type and mutant mitochondria and supported by
oxidation of substrates are compared by the histogram graph on the previous page. Open bars rep-
resent Wild Type mitochondria; cross-hatched bars represent the mutant mitochondria. Oxidation of
each of the substrates in the two graphs requires a coenzyme. On what…
A mutant haploid strain of Saccharomyces cerevisiae(yeast) called cox2-1 was found that was unable togrow on media containing glycerol as the sole sourceof carbon and energy. (Glycerol is a nonfermentablesubstrate for yeast.) This strain could, however, growon the fermentable substrate glucose. Researchers discovered that cox2-1 cells lack a mitochondrial proteincalled cytochrome c oxidase.a. Explain why cox2-1 cells can grow on mediumcontaining glucose but not on glycerol medium.
Chapter 15 Solutions
Genetics: From Genes to Genomes
Ch. 15 - Match each numbered item with the most closely...Ch. 15 - Assuming human cells have on average 1000...Ch. 15 - Reverse translation is a term given to the process...Ch. 15 - The human nuclear genome encodes tRNAs with 32...Ch. 15 - The human mitochondrial genome includes no genes...Ch. 15 - How do you know if the halibut you purchased at...Ch. 15 - Is each of these statements true of chloroplast or...Ch. 15 - Suppose you are characterizing the DNA of a...Ch. 15 - An example of a gene-targeting DNA plasmid vector...Ch. 15 - Which of the following characteristics of...
Ch. 15 - The Saccharomyces cerevisiae nuclear gene ARG8...Ch. 15 - The so-called hypervariable regions HV1 and HV2 of...Ch. 15 - Suppose a new mutation arises in a mitochondrial...Ch. 15 - Describe at least two ways in which the...Ch. 15 - Why are severe mitochondrial or chloroplast gene...Ch. 15 - Suppose you are examining a newly found plant...Ch. 15 - A form of male sterility in corn is inherited...Ch. 15 - Plant breeders have long appreciated the...Ch. 15 - A mutant haploid strain of Saccharomyces...Ch. 15 - Prob. 20PCh. 15 - What characteristics in a human pedigree suggest a...Ch. 15 - The first person in the family represented by the...Ch. 15 - In 1988, neurologists in Australia reported the...Ch. 15 - If you were a genetic counselor and had a patient...Ch. 15 - Kearns-Sayre syndrome KSS, Pearson syndrome, and...Ch. 15 - Many clinically relevant mitochondrial diseases...Ch. 15 - Leigh syndrome is characterized by psychomotor...Ch. 15 - All mutations in mitochondrial genes ultimately...Ch. 15 - How could researchers have determined that the...
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