This is a schematic diagram of an agarose gel used to analyze the DNA fragments generated by analyzing individual female flies with primers P1, P2, and P3 in a single PCR reaction. Lane M represents DNA fragments that are size markers (standards). In lanes 1, 2, and 3, sketch in the DNA fragments expected to be produced by PCR for female Drosophila homozygous for wild-type, heterozygous, and homozygous for the white-one mutation, respectively:

Q: Two homologous chromosomes in ES cells are depicted below with the portion of your gene of interest…

A: CRISPR/Cas9 creates specific double-stranded breaks at the target locus that trigger DNA repair…

Q: After much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2.  The…

A: CoB1 gene is a component of the ubiquinol- cytochrome c reductase complex that is part of the…

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: Select all that would be true if I had a missense mutation in an gene: The missense mutant protein…

A: A missense mutation is a type of point mutation in which a single nucleotide  change results in a…

Q: Clearly and neatly diagram an image of the agarose gel indicating the fragments that would be…

A: Electrophoresis It refers to the technique in which the fragments of molecules of DNA, RNA, or…

Q: Nikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a…

A: PCR or Polymerase Chain Reaction is a technique which is used to create several copies of a…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: vWA produce 200bp in PCR . All others produce less or more than 200 bp in PCR.  Here SSR means is…

Q: Both karyotyping and G_banding techniques show normal chromosomal pattern, however pedigree analysis…

A: Answer: Introduction: A pedigree shows the pattern of inheritance of a particular trait in entire a…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: What is the difference of intragenic and intergenic regions? Please explain with drawing.

A: In the intricate tapestry of the genome, the terms "intragenic" and "intergenic" encapsulate…

Q: You are attempting to genotype a series of cells at gene A through restriction enzyme digestion. You…

A: The restriction site mutation (RSM) assay is used to identify DNA mutations at common restriction…

Q: As you've learned, PCR stands for Polymerase Chain Reaction, and is a method for selecting and…

A: STR analysis It is a tool used in forensic analysis that evaluates specific short tandem repeats…

Q: In two independent PCR reactions, reaction A took 15 standard PCR cycles to obtain 1.31 × 108 copies…

A: PCR or the Polymerase Chain Reaction is a molecular technique widely used to syntheise multiple…

Q: How can you assess dominance and mapping with only one mutant?

A: Dominant alleles are those which are expressed when present in heterozygous conditions.  The

Q: In this module, you have learned about the use of balancer chromosomes (such as TM2 and TM6C in the…

A: Lethal genes are alleles that result in an end of the entity that possesses them. In basic terms,…

Q: If you wanted to make a mouse model for any of the following human genetic conditions (a–d),…

A: Since we only answer up to 3 sub-parts, we’ll answer the first three. Please resubmit the question…

Q: What is a fluorophore? If you wanted to fluorescently label a brain cell, describe one way you could…

A: Introduction  Brain cells, also known as neurons, are specialized cells that are the basic building…

Q: 22 22 22 16 22 7. Black Blue Purple Orange Green Gray | | | | || || || | || || || | || 8. | | || 7.…

A: Simple sequence repeats (SSRs) are also called microsatellite VNTR (variable number tandem repeat).…

Q: Here you see the gel electrophoresis of PCR reactions of the pMCT118 VNTR locus of 4 individuals…

A: Answer pMCT118 VNTR  locus has multiple repeats that can be analyzed by Polymerase chain reaction…

Q: Four E. coli strains of genotype atb¯ are labeled 1, 2, 3, and 4. Four strains of genotype a¯b* are…

A:

Q: parental and recombinant offsprings

A: Parental offsprings: These are the offsprings that have the same combination of alleles as one of…

Q: Why  do the two possible PCR products differ in size by  300 base  pairs?

A: In in vitro conditions, a polymerase chain reaction (PCR) is a technique for making several copies…

Q: overlapping one another and amplifying themselves. Where do you expect to see bands of Primer dimer…

A: Primer dimers are commonly caused due to contamination of our DNA sample and can be observed as a…

Q: Using the designed forward and reverse primer from Question 23-30, you performed PCR to amplify the…

A: The first step in designing a PCR primer to amplify the CO1 gene of S. tawilis and S. malengi is to…

Q: Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

A: The term SSR stands for simple sequence repeat that is a microsatellite VNTR (variable number tandem…

Q: Sequence length: 1000 The following sites are segregating: 12 A C G C T C G C C C G C C G G TTT G A…

A: A segregating site is a site in the DNA sequence of a population that shows variation in the…

Q: In our simple model of DNA amplification we assumed that a successful amplification doubled the DNA…

A: The question is asking us to consider a scenario where one allele in a sample is successfully…

Q: What is the probability that any random male in USA would share the same genotype as the murderer…

A: SSR (simple sequence repeats) or microsatellite refers to a tract of repetitive co-dominant…

Q: For your PCR reaction above, the primer set has a much higher AT (low GC) content than what wo be…

A: The content you provided discusses the concept of annealing temperature in the context of Polymerase…

Q: Primers can sometimes bind and target the wrong gene, especially if the primers are allowed to bind…

A: It is difficult for one to to find out gene of interest out of a complete genome. However, a given…

Q: Both karyotyping and G_banding techniques show normal chromosomal pattern, however pedigree analysis…

A: Cytogenetic approaches to studying chromosomes and their relationship to human disease have improved…

Q: Are you a hidden heterozygote? A PCR analysis (part2) Agarose gel electrophoresis and interpretation…

A: Agarose gel concentration  More the concentration of the agarose gel more smaller will be its pores.…

Q: The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted…

A: Hi, Thanks For Your Question.  Answer : Correct Option Is b (20%) Explanation :  P (III-7 Is…

Q: The restriction digests of the cloned Drosophila gene can provide direct visible evidence of a…

A: We can use the technique of Southern blotting to detect and isolate the DNA fragment with the Gene…

Q: Below is the DNA sequence (coding strand) for the human placental protein 14 (not all of the gene…

A: PCR stands for Polymerase Chain Reaction. It's a widely used laboratory technique in molecular…

Q: DNA polymorphisms can be used as DNA markers. Describe the differences between SNPs and STR…

A: DNA markers can help link an inherited disease with the responsible gene. the most common types of…

Q: Nikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a…

A: It is possible to amplify a particular DNA fragment using the molecular biology technique known as…

Q: A possible forward primer to be designed is a primer that binds to the region of the OXA-M290 gene…

A: Cloning is a fundamental technique in molecular biology that involves inserting a gene of interest…

Q: You are handling a paternity lawsuit brought against five poten woman. You isolated DNA from the…

A: Answer. DNA fingerprinting DNA fingerprinting also known as DNA typing or DNA profiling is a…

Q: You are studying a genome that has 42% G:C content and the remaining 58% of the genome is As and Ts.…

A: Restriction enzymes  The enzyme that is use to cut the DNA at specific site.

Q: What is inverse PCR? How are we going to use inverse PCR to help figure out the molecular location…

A: The gene is a basic physical and functional unit of heredity. Genes are made up of DNA. Some genes…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: Microsatellites, commonly referred to as Simple Sequence Repeats (SSRs), are brief repeated DNA…