(a) Interpretation: The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined. Concept introduction: The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 1

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Need help. A protein X binds Ligand Y. The kon characterizing this binding is 1 x 106 M-1s-1, koff is 2 x 10-3 M-1s-1. In your in vitro X-Y binding experiment, at what concentration of Ligand Y half of the Protein X would be bound to Y. Assume that the binding is according to the Lock-and-key model

Please help me with this question. PLEase give me the right answer and for number 2 WRITE AT LEAST A PARAGRAPGH and dont copy from internet.

SIGNALS AND TARGETS. Listed below are sample polypeptides/proteins with their signal molecule/peptide. Answer the questions that follow. If you are asked to give the amino acid sequence, provide the sequence using the three-letter names of the amino acids (eg. ser-ala-met). Catalase with H2N-...KERINGKERIANGEKSAMSKL-COOH What is the name of the specific receptor of this polypeptide? The receptor may also have what alternative function?

Answer 2

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 3

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 4

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 5

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 6

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 7

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 14 - Prob. 1P Ch. 14 - Prob. 2P Ch. 14 - Prob. 3P Ch. 14 - Prob. 4P Ch. 14 - Prob. 5P Ch. 14 - Prob. 6P Ch. 14 - Prob. 7P Ch. 14 - Prob. 8P Ch. 14 - Prob. 9P Ch. 14 - Prob. 10P

Solution Summary: The author explains that the ligand binding assay is an analytic procedure, in which the receptor binding is the basis of the experiment.

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Chapter 14 Solutions