The size of bands obtained after the cutting of DNA with Eco RI. Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

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Answer 1

Question

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

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You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an organism using the Hindill restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a. Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above,

A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?

See the restriction enzyme map below. The total DNA length is 1800 base pairs. If this DNA is cut using three restriction enzymes, namely Kpnl, Sall and EcoRI, it yields four fragments with sizes of 390 bR. 810 bp, 270 bp www and 330 bp. Kpnl Sall EcoRI 390 810 270 330 1800 bp 1. If you were to subject this digested DNA to agarose gel electrophoresis, what would your gel look like? Draw a detailed picture of your gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. 2. You are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exac fragment sizes are not important.

Answer 2

Question

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

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Answer 3

Question

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

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Answer 4

Question

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

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Answer 5

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 6

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 7

Chapter 13, Problem 9QP

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 8

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 9

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 10

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 11

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EG Ch. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EG Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CS Ch. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QP Ch. 13 - Prob. 3QP

Solution Summary: The author explains that every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence.

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