Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 4QP
Summary Introduction

Introduction: The recombinant organism shows the criteria of superiority from the parents. Involvement of genetic sequences takes place in the origination of the hybrid or recombinant organism.

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Part A:  During cloning, the DNA is cut with a restriction enzyme giving it what?   A. more introns   B. sticky ends   C.  a polyA tail   D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use?   A. PCR   B. epigenetics   C. hybridization   D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible?    A. transcription factor   B. rRNA   C. ribosome   D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what?   A. PCR   B. gene therapy   C. stem cell work   D. DNA fingerprinting
What is a cloning vector?   A. The DNA probe used to locate a particular gene in the genome.   B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.   C. The laboratory apparatus used to clone genes.         D.  An enzyme that cuts DNA into restriction fragments.
The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errors
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