The reasons that the presence of restriction enzymes inside the bacterial cell does not affect bacterial chromosomes. Introduction: A restriction enzyme is a form of a protein that is produced by bacteria. These enzymes are also termed as “restriction endonucleases.” The restriction enzyme plays an important role in the production of recombinant DNA.

Question

As you know, restriction enzymes evolved in different bacterial species independently. The adaptive significance of having a restriction enzyme is that the bacterium has the ability to cut the injected viral DNA into small segments. This destruction of viral DNA prevents the virus from taking over the bacterial cell and killing the cell. What is one benefit of using a restriction enzyme with staggered ends (such as EcoRI) to cut both the DNA insert and the plasmid? Which types of cut sites (staggered with “sticky ends” or blunt ends) are most useful in cloning DNA? Would you expect restriction enzymes in different bacteria genera (Streptococcus, Lactobacter, Escherichia) to have the same recognition sites (DNA sequences). Why or why not?

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Question

Chapter 13, Problem 6QP

Summary Introduction

To describe: The reasons that the presence of restriction enzymes inside the bacterial cell does not affect bacterial chromosomes.

Introduction: A restriction enzyme is a form of a protein that is produced by bacteria. These enzymes are also termed as “restriction endonucleases.” The restriction enzyme plays an important role in the production of recombinant DNA.

Summary Introduction

To explain: The reasons behind the presence of restriction enzymes inside the bacterial cell.

Introduction: A restriction enzyme is a form of a protein that is produced by bacteria. These enzymes are also termed as “restriction endonucleases.” The restriction enzyme plays an important role in the production of recombinant DNA.

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