Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 13, Problem 2AL
You have isolated several E. coli mutants:
Mutant #1 has a point mutation (a single base-pair change) in the −10 region of the promoter of a gene encoding an enzyme needed for synthesis of the amino acid serine.
Mutant #2 has a mutation in the −35 region in the promoter of the same gene.
Mutant #3 is a double mutant with mutations in both the −10 and −35 region of the promoter of the same gene.
Only Mutant #3 is unable to make serine. Why do you think this is so?
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A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the results shown below (the intact promoter is on
top). Based on these results, what conclusions could you draw from each of the deletion construct, and what do you know about the nature of the
promoter?
Relative activity
-500
Reporter gene
100%
Reporter gene
180%
-350
-250-
Reporter gene
180%
75%
-150
Reporter gene
45%
Reporter gene
-100
0%
- 8
Reporter gene
You have isolated a mutant that you call Mutant Z. In a Mutant Z strain, the RNA polymerase can
bind to the promoter, but it does not stay bound (e.g. it can only bind reversibly). A mutation in
which of the following cis elements could lead to the Mutant Z phenotype? (you may select more
than one option)
Oribosome binding site
O-35 promoter region
Odomain 2 of sigma
3/4 linker of sigma
-10 promoter region
Odomain 1 of sigma
Olac repressor
Odomain 4 of sigma
You made four mutants for a promoter sequence in DNA and studied them for transcription. The results of the amount of gene expression or transcription (based on beta-Gal activity shown on Y-axis) for these DNAs (X-axis) are shown. The sequence of the wild-type and mutant DNAs, and consensus sequence from many promoters are shown here for your convenience.
From this experiment you can conclude that:
Nucleotide substitution can identify important bases of the binding sites or promoter in DNA (e.g., -10 and -35 promoter sequences of lac operon).
True or false:
Spacer
(a)
-10 region
-35 region
TTGACA
Consensus sequence
TATAAT
Wild-type Lac promoter GGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT
Mutant 1 GGCTTTACACTTTATG-TTCCGGCTCGTATGTTGTGTGGAATT
Mutant 2 GGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATT
Mutant 3 GGCTTTACACTTTATG-TTCCGGCTCGTATAATGTGTGGAATT
Mutant 4 GGCTTGACACTTTATG-TTCCGGCTCGTATAATGTGTGGAATT
(b)
700
600-
500-
400-
300-
200-
100.
0
● True
O False
B-Galactosidase activity
Wild-type…
Chapter 13 Solutions
Prescott's Microbiology
Ch. 13.1 - MICRO INQUIRY Based on what we now know about...Ch. 13.1 - Prob. 2MICh. 13.1 - Retrieve, Infer, Apply 1. Briefly summarize the...Ch. 13.1 - Retrieve, Infer, Apply 2. Explain how protein was...Ch. 13.2 - MICRO INQUIRY To which carbon of ribose...Ch. 13.2 - MICRO INQUIRY How many H bonds are there between...Ch. 13.2 - Prob. 3MICh. 13.2 - Prob. 1CCCh. 13.2 - Retrieve, Infer, Apply What does it mean to say...Ch. 13.2 - Retrieve, Infer, Apply Amino acids are described...
Ch. 13.3 - MICRO INQUIRY What provides the energy to fuel...Ch. 13.3 - MICRO INQUIRY What is the difference between...Ch. 13.3 - MICRO INQUIRY Why cant DNA polymerase I perform...Ch. 13.3 - Retrieve, Infer, Apply How many replicons do...Ch. 13.3 - Prob. 2CCCh. 13.3 - Retrieve, Infer, Apply Describe the nature and...Ch. 13.3 - Retrieve, Infer, Apply Outline the steps Involved...Ch. 13.3 - Retrieve, Infer, Apply What is the end replication...Ch. 13.4 - Why is the nontemplate strand called the sense...Ch. 13.4 - Retrieve, Infer, Apply The coding region of a gene...Ch. 13.4 - Which strand of a gene has sequences that...Ch. 13.4 - Briefly discuss the general organization of tRNA...Ch. 13.5 - MICRO INQUIRY Are the -35 and -10 regions...Ch. 13.5 - Retrieve, Infer, Apply Outline the transcription...Ch. 13.5 - Retrieve, Infer, Apply What is a polycistronic...Ch. 13.5 - Retrieve, Infer, Apply What is a consensus...Ch. 13.5 - Tabulate the similarities and differences between...Ch. 13.6 - Prob. 1MICh. 13.6 - What is the difference between a codon and an...Ch. 13.6 - Prob. 2CCCh. 13.6 - Retrieve, Infer, Apply What is meant by code...Ch. 13.6 - Retrieve, Infer, Apply Is the genetic code truly...Ch. 13.7 - MICRO INQUIRY Why is simultaneous transcription...Ch. 13.7 - MICRO INQUIRY What would be the outcome if an...Ch. 13.7 - MICRO INQUIRY Why would it be impossible for...Ch. 13.7 - MICRO INQUIRY What provides the energy to fuel...Ch. 13.7 - Retrieve, Infer, Apply In which direction are...Ch. 13.7 - Retrieve, Infer, Apply Briefly describe the...Ch. 13.7 - Retrieve, Infer, Apply What are the translational...Ch. 13.7 - Prob. 4CCCh. 13.7 - Retrieve, Infer, Apply How many ATP and GTP...Ch. 13.8 - Prob. 1CCCh. 13.8 - Prob. 2CCCh. 13.8 - Retrieve, Infer, Apply Give the major...Ch. 13.8 - Prob. 4CCCh. 13 - Prob. 1RCCh. 13 - Prob. 2RCCh. 13 - Prob. 3RCCh. 13 - Prob. 4RCCh. 13 - Prob. 5RCCh. 13 - Streptomyces coelicolor has a linear chromosome....Ch. 13 - You have isolated several E. coli mutants: Mutant...Ch. 13 - Prob. 3ALCh. 13 - Prob. 4AL
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