Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12.1, Problem 2TQ
Summary Introduction
To review:
The strategy to construct a strain of Escherichia coli that cannot synthesize histidine.
Introduction:
Histidine biosynthesis is a pathway present in prokaryotes and lower eukaryotes. This pathway controls fundamental regulatory processes in these organisms. It is postulated that histidine is synthesized abiotically. The biosynthetic pathway of histidine contains 10 steps and is unbranched.
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A newly-identified relative of Staphylococcus is causing great concern at the Center for Disease Control. It has been observed that some strains of this new bacterial species are resistant to multiple antibiotics used in clinical settings. Thanks to your bacterial genetic prowess, you are hired as part of a research team whose mission is to characterize this new species. It appears that at least three different antibiotic resistance genes have integrated onto the bacterial chromosome:
min+ = confers resistance to minocycline
cep+ = confers resistance to cephalexin
van+ = confers resistance to vancomycin
The following table shows the results of your experiments. Transformation of the recipient cells was conducted, using the DNA of the donor cells. You next selected for recipient cells that had undergone transformation by plating the transformed cells on media containing minocycline. This enabled you to select for min+ cells. The min+ cells were then replica plated onto media…
In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f,
g, h and i pairs of genes were analyzed for co-transformation with the following results:
Gene Pair
Co-transformation
g+ i+
f+ i+
yes
no
f+ h+
Уes
f+ g+
no
g+ h+
h+ i+
no
уes
What is the linear order of these genes relative to cach other?
In yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what would happen to the telomeres over time in a yeast lineage in which the following mutations were created.
a) The gene encoding the catalytic subunit of the telomerase is deleted.
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Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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