Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12, Problem 10RQ
Summary Introduction
To review:
The description of the two-hybrid analysis and the bait and prey proteins.
Introduction:
To predict the function of a protein, protein-protein interaction plays a significant role. There are in vivo and in vitro methods to determine the interaction between proteins. The in vitro method is tandem affinity purification while the in vivo is the yeast two-hybrid system. Protein-protein interactions include
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Describe about fluorescence in situ hybridization (FISH).
describe fluorescent in situ hybridization(FISH)
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Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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- What is FISH? Describe an example in which the application of CARD-FISH might yield better results than traditional FISH.arrow_forwardExplain: Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved? functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe. Explain how bacteria are being detected using an electrochemical sensorarrow_forwardWhat are Short hybridization probes?arrow_forward
- Mark the alternative that best explains the strategies for using tails and/or fusion proteins associated with the protein of interest. * a) fusion proteins have different functions, including increasing the molecular weight of the protein of interest and thereby facilitating the process of protein purification by affinity chromatography. b) Histidine, c-Myc and GST tails have a dual function, since they can act both in reducing the formation of inclusion bodies and in facilitating the solubility of the protein of interest. c) the introduction of fusion proteins, such as Maltose-binding protein (MBP), at the C-terminus of the protein of interest has as main objective to increase the expression yield as they facilitate the protein aggregation process. d) the introduction of tails, such as c-Myc, in the C-terminus aims to facilitate the protein purification process through the affinity chromatography methodology.arrow_forwardWhat is FISH analysis?arrow_forwardHow would you simply explain FISH (Fluorescence in situ Hybridization)?arrow_forward
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