Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12, Problem 13RQ
Summary Introduction
To review:
The production of more toxic toxins by phage display and its ramifications.
Introduction:
Phage display techniques are used in the study of protein-protein, protein-peptide, and DNA-protein interaction. The peptides that bind to a specific target protein can also be revealed by using this technique. It can quickly find the variant with anticipated traits by screening millions of mutants. This technique helps the researchers to screen for the transformed properties of the peptide variants.
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Students have asked these similar questions
One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
About the technique of phage display:
MOLECULAR BIOLOGY_advanced
The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria.
Phages are selected if they express on their surface the optimized ligand.
One selects Escherichia coli cells that are resistant to the phage infection.
More than one optimized ligand can be selected during the panning procedure.
The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.
Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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- What does the acronym CRISPR stand for? Briefly outline the components of the CRISPR/Cas system. What is the function of the CRISPR/Cas system?What do you think about the ethical impacts of a technology such as CRISPR? What is the main parameter that is used to define new virus families among archaeal viruses? List and outline that various stages through which bacteria and archaea fight back against infection with viruses.arrow_forwardWhich of the following is not associated with gene therapy SCID patients A) utilizes an enzyme to repair the patient’s nonfunctioning ADA gene B) isolation of patient cells C) cloning of functioning a D a gene in bacteria D) use of vectorarrow_forwardGeneralized transduction can transfer: please explain the answer a.any DNA from the host genome b.only phage-specific DNA sequences c.genes flanked by insertion elements d.only genes near the site of integrationarrow_forward
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