Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12.1, Problem 3TQ
Summary Introduction
To review:
The experiments which should be done to identify whether the control is transcriptional or translational.
Introduction:
During unfavourable conditions, the cell can regulate its gene expression by altering the rate of transcription and translation or by altering the stability of mRNA (messenger RNA) and proteins. In order to determine the change in the amount of a specific RNA (ribonucleic acid) transcript, quantitative PCR (polymerase chain reaction) can be performed.
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The amino acid asparagine is synthesized from aspartic acid by the enzyme asparagine synthetase (AS).
In the previous problem you proposed a model for how this gene could be regulated. Suppose that you carry out an experiment to test your model. To do this you cut out the regulatory sequences upstream of the gene and fuse it to a gene for green fluorescent protein (GFP). Now you can visually observe when the gene is activated. You insert this engineered gene into a host cell and look for GFP expression. You discover some mutants that have different expression levels of GFP and call them GFP1- and GFP2-. The expression levels of GFP are given below.
Cell
GFP expression
Wild type
100
GFP1-
50
GFP2-
0
Propose an explanation for these results based on your model. In other words, what was mutated and how?
Your answer should include whether the mutation is (see links for more information):
dominant or recessive https://www.ncbi.nlm.nih.gov/books/NBK21578/#A1877…
The amino acid asparagine is synthesized from aspartic acid by the enzyme asparagine synthetase (AS).
In the previous problem you proposed a model for how this gene could be regulated. Suppose that you carry out an experiment to test your model. To do this you cut out the regulatory sequences upstream of the gene and fuse it to a gene for green fluorescent protein (GFP). Now you can visually observe when the gene is activated. You insert this engineered gene into a host cell and look for GFP expression. You discover some mutants that have different expression levels of GFP and call them GFP1- and GFP2-. The expression levels of GFP are given below.
Cell
GFP expression
Wild type
100
GFP1-
50
GFP2-
0
Propose an explanation for these results based on your model. In other words, what was mutated and how?
This answer should include whether the mutation is (view links for more information):
dominant or recessive https://www.ncbi.nlm.nih.gov/books/NBK21578/#A1877
in a cis…
Consider the following simple regulatory pathways. Assume the full pathway is shown.
A-
E-
B-
F-
C-
G-
D-
1
H-
A
You identify several null mutations (a complete deletion of the gene). For each mutant (ind
with a - sign), determine whether the final product (I, J, K or L) is inducible, uninducible, or
constitutive.
2 B
3 C
4 D
inducible
inducible
constitutive
uninducible
constitutive
inducible
inducible
E
uninducible
F
G
H
>
>
>
Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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