Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 12, Problem 9TYU
Topoisomerases (a) synthesize DNA (b) synthesize RNA primers (c) join Okazaki fragments (d) break and rejoin DNA to reduce torsional strain (e) prevent single DNA strands from joining to form a double helix
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Match each enzyme name in the left column with the correct descriptive phrase in the right column.
(a) Topoisomerase II(b) DNA ligase(c) DNA polymerase γ(d) Reverse transcriptase(e) DNA polymerase I(f) DNA polymerase III
i. Catalyzes most nucleotide incorporations in bacterial DNA replicationii. Cleaves RNA in a DNA–RNA hybrid moleculeiii. Uses a tRNA primer in synthesis of retroviral DNAiv. Acts through an adenylylated DNA intermediatev. Catalyzes formation of a double-strand DNA breakvi. Catalyzes mitochondrial DNA replication
During DNA replication, topoisomerase breaks
(a) peptide bonds (b) disulfide bonds (c) phosphodiester bonds (d) ionic bonds (e) hydrogen bonds
The problem of replicating the lagging strand—that is, adding bases in the 3’ to 5’ direction—is solved by DNA through the use of
(a) base pairing (b) replication forks (c) helicase (d) Okazaki fragments (e) topoisomerase
Chapter 12 Solutions
Biology (MindTap Course List)
Ch. 12.1 - Summarize the evidence that accumulated during the...Ch. 12.1 - Prob. 2LOCh. 12.1 - Prob. 1CCh. 12.1 - Prob. 2CCh. 12.2 - Explain how nucleotide subunits link to form a...Ch. 12.2 - Describe how the two strands of DNA are oriented...Ch. 12.2 - Prob. 5LOCh. 12.2 - Prob. 1CCh. 12.2 - Prob. 2CCh. 12.2 - Prob. 3C
Ch. 12.3 - Cite evidence from Meselson and Stahls experiment...Ch. 12.3 - Prob. 7LOCh. 12.3 - Explain the complexities of DNA replication that...Ch. 12.3 - Discuss how enzymes proofread and repair errors in...Ch. 12.3 - Prob. 10LOCh. 12.3 - How did the ability to distinguish old and newly...Ch. 12.3 - What feature of DNA structure causes DNA...Ch. 12.3 - What is the reason that eukaryotic cells require...Ch. 12 - When Griffith injected mice with a combination of...Ch. 12 - Which of the following inspired Avery and his...Ch. 12 - In the Hershey-Chase experiment with...Ch. 12 - The two complementary strands of the DNA double...Ch. 12 - If a segment of DNA is 5 CATTAC 3, the...Ch. 12 - Each DNA strand has a backbone that consists of...Ch. 12 - The experiments in which Meselson and Stahl grew...Ch. 12 - The statement DNA replicates by a semiconservative...Ch. 12 - Topoisomerases (a) synthesize DNA (b) synthesize...Ch. 12 - A lagging strand forms by (a) joining primers (b)...Ch. 12 - The immediate source of energy for DNA replication...Ch. 12 - Which of the following statements about eukaryotic...Ch. 12 - Prob. 13TYUCh. 12 - Prob. 14TYUCh. 12 - Prob. 15TYUCh. 12 - INTERPRET DATA In the Hershey-Chase experiment,...Ch. 12 - EVOLUTION LINK How does DNA being the universal...
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- The statement “DNA replicates by a semiconservative mechanism” means that (a) only one DNA strand is copied (b) first one DNA strand is copied and then the other strand is copied (c) the two strands of a double helix have identical base sequences (d) some portions of a single DNA strand are old and other portions are newly synthesized (e) each double helix consists of one old and one newly synthesized strandarrow_forwardWithout the action of DNA ligase, cells would not be ableto complete their replication. What is the function of DNAligase?(a) Unzips the DNA double helix(b) Stabilizes single-stranded DNA(c) Binds DNA sequences together to generate acontinuous strand(d) Proofreads the replication process(e) Creates an RNA copy of the DNAarrow_forwardThe process of DNA replication is affected by an enzyme known as: (a) Mutase (b) Ligase (c) Polymerase I (d) Ribonucleasearrow_forward
- Which of the following best explains the production of Okazaki fragments in replicating DNA (a) DNA is stressed when it unwinds (b) DNA is anti-parallel and can only be synthesized 5’ to 3’ (c) DNA contains once less oxygen in its sugar while RNA has an OH attached to its 2’ carbon (d) Template strands are complementary and have a tendency to reform hydrogen bonds (e) both a and darrow_forwardThe elongation of the leading strand during DNA synthesis(A) progresses away from the replication fork.(B) occurs in the 3′ S 5′ direction.(C) produces Okazaki fragments.(D) depends on the action of DNA polymerasearrow_forwardThe experiments in which Meselson and Stahl grew bacteria in heavy nitrogen conclusively demonstrated that DNA (a) is a double helix (b) replicates semiconservatively (c) consists of repeating nucleotide subunits (d) has complementary base pairing (e) is always synthesized in the 5'---->3' directionarrow_forward
- 5) In DNA replication, which of the following events happens during both leading and lagging strand synthesis? a. RNA primers help initiate DNA synthesis. b. Topoisomerase separates complementary strands of DNA. c. Ligase joins together multiple Okazaki fragments. d. DNA polymerase synthesizes a single, continuous strand of DNA. Explain WHY? 6) Suppose you are provided with an actively dividing culture of E. coli bacteria to which radioactive thymine has been added. What would happen if a cell replicates once in the presence of this radioactive base? a. One of the daughter cells, but not the other, would have radioactive DNA. b. Neither of the two daughter cells would be radioactive. c. All four bases of the DNA would be radioactive. d. DNA in both daughter cells would be radioactive. Explain WHY?arrow_forwardThe two complementary strands of the DNA double helix are held to each other by (a) ionic bonds between deoxyribose molecules (b) ionic bonds between phosphate groups (c) covalent bonds between nucleotide bases (d) covalent bonds between deoxyribose molecules (e) hydrogen bondsbetween nucleotide basesarrow_forwardThe following diagram represents a DNA molecule that is undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forward
- The following diagrams represent DNA molecules that are undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forward(1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA? (4) Which nucleic acid is probably depicted here? (5) What are these short DNA fragments usually called? (6) Which enzyme probably functions here to couple these two newly synthesized fragments of DNA? (7) Is this strand the leading or lagging strand? (8) Which end (5' or 3') of the molecule is here?arrow_forward(a) Eukaryotic DNA replication is more complex than prokaryotic replication. Give one reason why this should be so. (b) Why might eukaryotic cells need more kinds of DNA polymerases than bacteria?arrow_forward
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