BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
3rd Edition
ISBN: 9781260670929
Author: Hoefnagels
Publisher: MCG CUSTOM
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Chapter 11, Problem 5WIO
Summary Introduction
To determine:
The techniques used in DNA technology by the researchers to reveal the origin of meat.
Introduction:
DNA technology is the study and altering the genetic material of an organism to produce a new trait. The technology is useful in producing the pest resistance crops, highly nutritional food and so on. DNA technology consists of various techniques like Recombinant DNA technology, Gene therapy, DNA fingerprinting, DNA microarray technique and many more.
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Below is an image of the results of a gel electrophoresis experiment.
Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the
DNA size marker (DSM). From this image and by only using Lanes 1-
4 which lane matches each description below?
a) This lane contains the largest DNA fragment:
b) This lane contains the smallest DNA fragment:
c) This lane contains a 2.5 kbp DNA fragment:
42
| ||
||| | ||
12 kb
9.5 kb
6.5 kb
4 kb
2 kb
***
Which of the following statements regarding DNA fingerprinting is false?a) DNA fingerprinting cannot be used for paternity testingb) DNA profile using STR (short terminal repeats) is unique to an individualc) PCR is used for DNA profilingd) Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individuals
8) The majority of direct-to-consumer genetic tests:
A) must be obtained through a doctor
B) makes medical diagnosis directly available to people
C) are only available for conditions in which the test results are highly conclusive and reliable.
D) analyzes associations found between particular gene sequences and increased risk for conditions
Chapter 11 Solutions
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Ch. 11.1 - What is DNA technology?Ch. 11.1 - In what fields is DNA technology useful?Ch. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - What are the steps in producing a transgenic...Ch. 11.2 - How do tagged nucleotides participate in first...Ch. 11.2 - What is the function of 98.5 of the human genome...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - How are short tandem repeats used in DNA...Ch. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Describe the differences among embryonic, adult,...
Ch. 11.3 - What are the potential medical benefits of stem...Ch. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Why is the technique used to clone mammals called...Ch. 11.4 - Explain how and why a researcher might use a DNA...Ch. 11.4 - Compare and contrast preimplantation genetic...Ch. 11.4 - Prob. 3MCCh. 11.4 - Describe how CRISPR-Cas9 targets a specific gene...Ch. 11.4 - Prob. 5MCCh. 11 - If a restriction enzyme cuts between G and A...Ch. 11 - Which of the following is not a reason that...Ch. 11 - The function of electrophoresis is to a. break a...Ch. 11 - Why is PCR useful? a. Because it replicates all...Ch. 11 - Suppose an investigator at the scene of a murder...Ch. 11 - What is an induced pluripotent stem cell? a. A...Ch. 11 - Dolly the sheep was the first clone of an adult...Ch. 11 - Prob. 8MCQCh. 11 - Preimplantation genetic diagnosis would be least...Ch. 11 - What is the role of a virus in gene therapy? a. It...Ch. 11 - What techniques might researchers use to produce...Ch. 11 - Transgenic crops often require fewer herbicides...Ch. 11 - Describe why sorting DNA fragments by size is...Ch. 11 - Explain how the ingredients in a PCR reaction tube...Ch. 11 - Prob. 5WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - Prob. 7WIOCh. 11 - Mature neurons in the brain do not replicate. Why...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Scientists are interested in cloning an extinct...Ch. 11 - Prob. 11WIOCh. 11 - Prob. 12WIOCh. 11 - Use the Internet to research an application of...Ch. 11 - Prob. 14WIOCh. 11 - Review Burning Question 11.11, which describes the...Ch. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR related to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...Ch. 11 - How is a patient who receives gene therapy similar...
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- Please answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardWhich of the following statements concerning recombinantDNA technology is false?(a) Thus far, no illnesses in laboratory workers have beentraced to genetic recombinants.(b) Production of large amounts of proteins such as insulinand human growth hormone has been made possible us-ing recombinant DNA technology.(c) Recombinant DNA technology offers specific benefitsto the scientific, medical, and general populations.(d) Mutant strains of bacteria produced by genetic re-combination are often unable to survive in the naturalenvironment.(e) Recombinant DNA technology provides a high degreeof risk to the health of the general populations.arrow_forwardScientists have reportedly isolated short fragments of DNA from fossilized dinosaur bones hundreds of millions of years old. The technique used to isolate this DNA is the polymerase chain reaction, which is capable of amplifying very small amounts of DNA a millionfold. Critics have claimed that the DNA isolated from dinosaur bones is not purely of ancient origin, but instead has been contaminated by DNA from present-day organisms such as bacteria, mold, or humans. What precautions, analyses, and control experiments could be carried out to ensure that DNA recovered from fossils is truly of ancient origin?arrow_forward
- You purified the genome of the coliphage X174. After analysis, you find that its composition is 25 percent A, 33 percent T, 24 percent G, and 18 percent C. Based on this finding, which of the following prediction would you make for the coliphage $X174? The genome of coliphage X174 is double-stranded DNA. The genome of coliphage X174 is single-stranded DNA. The genome of coliphage X174 is single-stranded RNA. The genome of coliphage $X174 is double-stranded RNA. Previous Page Next Page Page 9 of 30arrow_forwardb ) Describe the contribution of Griffith , Avery et al . , Hershey & Chase , and Watson and Crick in the field of genetics . You are characterizing a newly discovered organism . You have noticed that the organism grows on two carbon sources - sucrose and mannitol . Whenever you add both sugars to the medium , sucrose will be completely metabolized before mannitol . a ) What is this growth pattern called ? b ) Describe a possible growth curve of sequential metabolism of the sugars . arrow_forwardFor what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA fragments C) to identify individuals who have committed crimes D) to identify single nucleotide polymerasearrow_forward
- Is the DNA you extracted is pure? What are the possible impurities? What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this. Imagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.arrow_forward1.) What characteristics of VNTR and STR make them useful for DNA fingerprinting? 2.) How does PCR minimize the problems associated with degraded DNA? 3.) What factors can cause DNA to become degraded? 4.) If Ethidium bromide was not added to a gel, what would happen? 5.) How can you tell if an individual is heterozygous for the D1S80 marker? 6.) If a negative control produces a band, what does this indicate? 7.) In an experiment, a student’s sample amplified for D1S80 produced 3 bands. It was the only DNA sample run on the gel. The student knows that there was no problem with the Thermocycler or primers because the other students in the class had the expected results of only one or two bands. What is the most likely explanation for these results?arrow_forwardGiven the situation: A paleontologist has recovered a tiny bit of organic material fromthe 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds.Which of the following would be most useful for increasing theamount of DNA available for testing? Option a.) restriction fragment analysis Option b.) polymerase chain reaction Option c.) electrophoresisarrow_forward
- Please answer these two questions regarding PCR a) First question you must use the image: What is the DNA profile for suspect B for the TH01 locus? b) What determines the target sequence amplified in PCR?arrow_forwardWhich of the following pairs of sequences would be considered different alleles in DNA profiling? a) ATGAATTCGG; ATGAAATCGG b) ATGAATTCGG; TACTTACTTACT c) GAAGAAGAA; GAAGAAGAAGAA d) AATAATAATAAT; AATTAATTAATTarrow_forwardImagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.arrow_forward
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