BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
3rd Edition
ISBN: 9781260670929
Author: Hoefnagels
Publisher: MCG CUSTOM
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Textbook Question
Chapter 11, Problem 4MCQ
Why is PCR useful?
| a. | Because it replicates all the DNA in a cell |
| b. | Because it can produce large amounts of DNA from small amounts |
| c. | Because it produces a heat-tolerant DNA polymerase |
| d. | Because it occurs in an automated device |
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Students have asked these similar questions
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR?
A. PCR is more likely to have errors introduced in the copying process.
B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR.
C. PCR can only be conducted in eukaryotic cells.
D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
Would it be possible to use human polymerase for the PCR reaction?
a.
No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur.
b.
No, because human polymerase cannot be extracted from cells to use in a lab setting.
c.
Yes, because we are using human DNA as the template DNA.
d.
Yes, because human polymerase can add bases to a template strand without a primer.
Chapter 11 Solutions
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Ch. 11.1 - What is DNA technology?Ch. 11.1 - In what fields is DNA technology useful?Ch. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - What are the steps in producing a transgenic...Ch. 11.2 - How do tagged nucleotides participate in first...Ch. 11.2 - What is the function of 98.5 of the human genome...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - How are short tandem repeats used in DNA...Ch. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Describe the differences among embryonic, adult,...
Ch. 11.3 - What are the potential medical benefits of stem...Ch. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Why is the technique used to clone mammals called...Ch. 11.4 - Explain how and why a researcher might use a DNA...Ch. 11.4 - Compare and contrast preimplantation genetic...Ch. 11.4 - Prob. 3MCCh. 11.4 - Describe how CRISPR-Cas9 targets a specific gene...Ch. 11.4 - Prob. 5MCCh. 11 - If a restriction enzyme cuts between G and A...Ch. 11 - Which of the following is not a reason that...Ch. 11 - The function of electrophoresis is to a. break a...Ch. 11 - Why is PCR useful? a. Because it replicates all...Ch. 11 - Suppose an investigator at the scene of a murder...Ch. 11 - What is an induced pluripotent stem cell? a. A...Ch. 11 - Dolly the sheep was the first clone of an adult...Ch. 11 - Prob. 8MCQCh. 11 - Preimplantation genetic diagnosis would be least...Ch. 11 - What is the role of a virus in gene therapy? a. It...Ch. 11 - What techniques might researchers use to produce...Ch. 11 - Transgenic crops often require fewer herbicides...Ch. 11 - Describe why sorting DNA fragments by size is...Ch. 11 - Explain how the ingredients in a PCR reaction tube...Ch. 11 - Prob. 5WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - Prob. 7WIOCh. 11 - Mature neurons in the brain do not replicate. Why...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Scientists are interested in cloning an extinct...Ch. 11 - Prob. 11WIOCh. 11 - Prob. 12WIOCh. 11 - Use the Internet to research an application of...Ch. 11 - Prob. 14WIOCh. 11 - Review Burning Question 11.11, which describes the...Ch. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR related to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...Ch. 11 - How is a patient who receives gene therapy similar...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardPCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forward
- Transcriptomes, that is, all the transcripts in a cell, are analyzed using: a. a type of PCR b. fluorescently labeled nucleotides c. microarrays d. all the abovearrow_forward1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)arrow_forwardPredict the effect on the PCR reaction if any of the following circumstances arose: i) there are no primers in the reaction, i) there are no dNTPs in the reaction, iii) there is no Taq polymerase in the reaction, A PCR would not commence. B. PCR would proceed normally. C. PCR would cease after a few cycles. D. Non-specific DNA amplification will occur.arrow_forward
- What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forward
- If a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles? a. About 100 b. About 1000 c. About 10,000 d. About 1010arrow_forwardWhat are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardHuman growth hormone is being researched as a potential anti-aging drug. What is the most likely way that would scientists produce large amounts of growth hormone? O a. Use transcription factors to increase the rate of transcription of the relevant gene in humans Use the human growth hormone to develop an aging vaccine Use restriction enzymes to isolate the relevant gene and allow it to replicate in all types of cells O b. O c. O d. Transfer the relevant gene to a bacterial cell and culture large colonies then purify the product Oarrow_forward
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