BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
3rd Edition
ISBN: 9781260670929
Author: Hoefnagels
Publisher: MCG CUSTOM
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Textbook Question
Chapter 11, Problem 1PIT
Review the Survey the Landscape figure in the chapter introduction. Given DNA’s role in the cell, why do the basic tools of DNA technology summarized in figure 11.17 have applications in such diverse fields of study?
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Please read the scenario below and then answer the question (in bold) that follows.
An original DNA sequence reads 3'- GGCGCTAGAATC -5'. A mutated version of that DNA strand is produced that reads 3-GGCTCTAGAATC -5.
Based on the information above, how severe of an impact do you believe the mutation would have on the cell (and therefore individual) in which the mutation occurred?
O a. Very severe, because the mutation leads to the formation of a truncated polypeptide
O b. Moderately to very severe, because the mutation results in the formation of a polypeptide that contains amino acids not found in the unmutated polypeptide
O c. Likely not that severe, because the amino acid sequence of the mutated polypeptide is identical to the amino acid sequence of the nonmutated polypeptide
List and describe the processes that make up the central dogma of biology which provides the path for the flow of genetic information from DNA to the proteins that are synthesized and carry out the functions needed by the cell.
Researchers can isolate a sample of cells, such as skin fibroblasts, and grow them in the laboratory. This procedure is called a cell culture. A cell culture can be exposed to a sample of DNA. If the cells are treated with agents that make their membranes permeable to DNA, the cells may take up the DNA and incorporate it into their chromosomes. This process is called transformation or transfection. Scientists have transformed human skin fibroblasts with methylated DNA and then allowed the fibroblasts to divide for several cellular generations. The DNA in the daughter cells was then isolated, and the segment that corresponded to the transformed DNA was examined. This DNA segment in the daughter cells was also found to be methylated. However, if the original skin fibroblasts were transformed with unmethylated DNA, the DNA found in the daughter cells was also unmethylated. With regard to the transformed DNA, do fibroblasts perform de novo methylation, maintenance methylation, or both?…
Chapter 11 Solutions
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Ch. 11.1 - What is DNA technology?Ch. 11.1 - In what fields is DNA technology useful?Ch. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - What are the steps in producing a transgenic...Ch. 11.2 - How do tagged nucleotides participate in first...Ch. 11.2 - What is the function of 98.5 of the human genome...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - How are short tandem repeats used in DNA...Ch. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Describe the differences among embryonic, adult,...
Ch. 11.3 - What are the potential medical benefits of stem...Ch. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Why is the technique used to clone mammals called...Ch. 11.4 - Explain how and why a researcher might use a DNA...Ch. 11.4 - Compare and contrast preimplantation genetic...Ch. 11.4 - Prob. 3MCCh. 11.4 - Describe how CRISPR-Cas9 targets a specific gene...Ch. 11.4 - Prob. 5MCCh. 11 - If a restriction enzyme cuts between G and A...Ch. 11 - Which of the following is not a reason that...Ch. 11 - The function of electrophoresis is to a. break a...Ch. 11 - Why is PCR useful? a. Because it replicates all...Ch. 11 - Suppose an investigator at the scene of a murder...Ch. 11 - What is an induced pluripotent stem cell? a. A...Ch. 11 - Dolly the sheep was the first clone of an adult...Ch. 11 - Prob. 8MCQCh. 11 - Preimplantation genetic diagnosis would be least...Ch. 11 - What is the role of a virus in gene therapy? a. It...Ch. 11 - What techniques might researchers use to produce...Ch. 11 - Transgenic crops often require fewer herbicides...Ch. 11 - Describe why sorting DNA fragments by size is...Ch. 11 - Explain how the ingredients in a PCR reaction tube...Ch. 11 - Prob. 5WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - Prob. 7WIOCh. 11 - Mature neurons in the brain do not replicate. Why...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Scientists are interested in cloning an extinct...Ch. 11 - Prob. 11WIOCh. 11 - Prob. 12WIOCh. 11 - Use the Internet to research an application of...Ch. 11 - Prob. 14WIOCh. 11 - Review Burning Question 11.11, which describes the...Ch. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR related to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...Ch. 11 - How is a patient who receives gene therapy similar...
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- Using the figure below identify: What is a function of introns and exons? What is a role of mobile DNA elements? What is a meaning of simple-sequence DNA?arrow_forwardAnswer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger printing. n) What is gene cloning? o) Elaborately…arrow_forwardThere are three basic steps of DNA extraction: cell lysis, separation, and extraction. In the procedure for extracting DNA from human cheek cells, which parts belong to or explain the aforementioned three basic steps? You may refer to the following procedure: http://www.planet-science.com/categories/experiments/biology/2012/03/extract-your-own-dna.aspxarrow_forward
- Use the following information to answer the next question In the process of genetic engineering, recombinant DNA is produced by combining genetic material from two different sources. For this technique, genetic engineers are especially interested in a specific group of enzymes called restriction endonuclease. These enzymes are preferred because they join the DNA fragments at 3' ends join the DNA fragments at 5' ends cut DNA at specific sites within the DNA cut DNA from their 5' or 3' endsarrow_forwardIs cloning a form of genetic engineering? Explain your answerarrow_forwardPlease answer all of these questions in details Answer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger…arrow_forward
- Briefly explain about computer-automated high-throughput DNA sequencing ?arrow_forwardPlace the following steps in order to outline how enzymes are involved with proofreading newly formed DNA molecules. 1. DNA polymerase does not detect damaged DNA. 2. Ligase connects the free end of the new DNA with the old DNA. 3. DNA polymerase replaces the damaged DNA with the correct nucleotide. 4. Exonuclease cuts the damaged DNA strand in order to remove the damaged section.arrow_forwardOnce separated from other cellular components, extracted DNA can be used for a variety of purposes. Name 1 use of human DNA that is common today.arrow_forward
- Explain how cells activate nucleic acids for polymerization. Explain why DNA is stable and why its structure dictates its replication mechanism. Explain why many RNA molecules exhibit tertiary structure, while most DNA molecules do not. Explain how DNA replication occurs from structural and enzymatic perspectives. Develop an understanding of nucleic acid biology outside a natural biological context (such as PCR, etc.)arrow_forwardSuppose that you are given a short fragment of DNA to sequence. You amplify the fragment with PCR and set up a series of four dideoxy reactions. You then separate the products of the reactions by gel electrophoresis and obtain the following banding pattern:arrow_forwardChoose the combination of answers that most accurately completes the statement.The function of ligase is to a. rejoin segments of DNA c. synthesize cDNA b. make longitudinal cuts in DNA d. break down ligamentsarrow_forward
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