BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
3rd Edition
ISBN: 9781260670929
Author: Hoefnagels
Publisher: MCG CUSTOM
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Textbook Question
Chapter 11, Problem 3PIT
Add the terms restriction enzyme, plasmid, virus, DNA polymerase and short tandem repeat to this concept map.
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Students have asked these similar questions
Describe the structure of a bacterial genome, and explain how it differs from a
eukaryotic genome.
Explain what a plasmid is, and describe the role of plasmids in the spread of
antibiotic resistance genes.
Explain how bidirectional semiconservative DNA replication copies the circular
chromosome of a bacterium during cell division.
Explain the steps of replication by DNA polymerase, including initiation,
elongation, and termination.
Explain the different kinds of mutations and how they occur.
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.
Study the diagram below. Put the number of the step in the diagram by its description in the list of
steps at the bottom, which are not in the correct order. Then, answer the questions that follow.
Human Cell
2
5
4
1
Sticky
ends
Bacterial Cell
Bacterial
chromosome
3
Recombinant
DNA
Plasmid
Recombinant DNA is formed by mixing the
donor gene with the plasmid
Bacteria cell is screened & cloned (not
shown)
Plasmid put back into target (bacterial) cell
Desired gene (for human insulin) is cut from
donor DNA using restriction enzymes
DNA is extracted from donor cell (human)
Plasmid is removed from bacterial cell and
cut open using restriction enzymes
Chapter 11 Solutions
BIOLOGY:THE ESSENTIALS (LL) W/CONNECT
Ch. 11.1 - What is DNA technology?Ch. 11.1 - In what fields is DNA technology useful?Ch. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - What are the steps in producing a transgenic...Ch. 11.2 - How do tagged nucleotides participate in first...Ch. 11.2 - What is the function of 98.5 of the human genome...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - How are short tandem repeats used in DNA...Ch. 11.2 - Why do investigators sometimes analyze...Ch. 11.3 - Describe the differences among embryonic, adult,...
Ch. 11.3 - What are the potential medical benefits of stem...Ch. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Why is the technique used to clone mammals called...Ch. 11.4 - Explain how and why a researcher might use a DNA...Ch. 11.4 - Compare and contrast preimplantation genetic...Ch. 11.4 - Prob. 3MCCh. 11.4 - Describe how CRISPR-Cas9 targets a specific gene...Ch. 11.4 - Prob. 5MCCh. 11 - If a restriction enzyme cuts between G and A...Ch. 11 - Which of the following is not a reason that...Ch. 11 - The function of electrophoresis is to a. break a...Ch. 11 - Why is PCR useful? a. Because it replicates all...Ch. 11 - Suppose an investigator at the scene of a murder...Ch. 11 - What is an induced pluripotent stem cell? a. A...Ch. 11 - Dolly the sheep was the first clone of an adult...Ch. 11 - Prob. 8MCQCh. 11 - Preimplantation genetic diagnosis would be least...Ch. 11 - What is the role of a virus in gene therapy? a. It...Ch. 11 - What techniques might researchers use to produce...Ch. 11 - Transgenic crops often require fewer herbicides...Ch. 11 - Describe why sorting DNA fragments by size is...Ch. 11 - Explain how the ingredients in a PCR reaction tube...Ch. 11 - Prob. 5WIOCh. 11 - Why are entire genomes not used for DNA profiling?Ch. 11 - Prob. 7WIOCh. 11 - Mature neurons in the brain do not replicate. Why...Ch. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - Scientists are interested in cloning an extinct...Ch. 11 - Prob. 11WIOCh. 11 - Prob. 12WIOCh. 11 - Use the Internet to research an application of...Ch. 11 - Prob. 14WIOCh. 11 - Review Burning Question 11.11, which describes the...Ch. 11 - Review the Survey the Landscape figure in the...Ch. 11 - How does PCR related to DNA profiling and...Ch. 11 - Add the terms restriction enzyme, plasmid, virus,...Ch. 11 - How is a patient who receives gene therapy similar...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Draw a figure showing annealing and extension of the oligos to form the DNA template.Label the following parts on your diagram: T7 Promoter, GG, Target Sequence, Tail Overlap, Tail. Explain the purpose of each labeled partarrow_forwardExplain why the genes whose products are required for the normal growth of bacteria not carried on plasmids? Give two examples (one bacterial gene and one plasmid gene) to support your answer.arrow_forwardcreate a concept map illustrating the relationships among these key terms: 1. Foreign DNA; 2. Restriction endonuclease; 3. Ligase; 4. Plasmid; 5. Origin of replication; 6. Recombinant Plasmid;7. Multiple cloning sites.arrow_forward
- Using a flow diagram, elaborate on how you would generate a recombinant plasmid.arrow_forwardUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. If the Nco1/EcoRI double digests produces 3 fragments what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb, 500 b and 500 b 3.0 kb, 1.0kb and 1.0 kbarrow_forwardSome DNA vaccines use a brief and small electrical shock to get DNA in plasmids into cells. What advantage would there be in using DNA vaccines that consist of plasmids instead of just pieces of double-stranded DNA? The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the spike protein of the virus. The virus uses the spike protein to invade human cells where it replicates. Is it surprising that the mRNA must be stabilized with chemicals that need ultra-cold or frozen storage to protect the mRNA from degradation before it causes human muscle cells to make the spike protein? Why not just inject the double-stranded cDNA that codes for the spike protein of the virus? What additional step or steps would you need to use to get the human muscle cells to produce the spike protein if the cDNA was injected to serve as the virus?arrow_forward
- Part A How is a gene for a particular protein inserted into a plasmid? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. phosphate groups sticky ends an apurinic enzyme nitrogen-containing bases a recombinant DNA joined Submit cut ovide Feedback a donor DNA a restriction enzyme Request Answer When a gene has been obtained using Reset Help it is mixed with the plasmids that have been by the same enzyme. When mixed together in a fresh E. coli culture, the of the DNA fragments bond with the of the plasmid DNA to formarrow_forwardDefine the following words: replication fork, leading strand, lagging strand, Okazaki fragments, helicase, single strand, binding protein, primase, DNA polymerase 3, DNA polymerase 1, dna ligase and nucleasearrow_forwardDifferentiate between TWO of the following pairs: Genetic and a restriction map Southern and colony blotting Polymerase chain reaction (PCR) and cloningarrow_forward
- Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it producesarrow_forwardUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kbarrow_forwardA plasmid is a DNA double helix in which the ends of each of the strands of nucleotides are attached to each other, forming a circular DNA molecule. 1) True 2) Falsearrow_forward
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