How is a gene for a particular protein inserted into a plasmid? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. phosphate groups sticky ends an apurinic enzyme nitrogen-containing bases Submit a recombinant DNA joined cut a donor DNA a restriction enzyme Request Answer When a gene has been obtained using Reset Help it is mixed with the plasmids that have been by the same enzyme. When mixed together in a fresh E. coli culture, the of the DNA fragments bond with the of the plasmid DNA to form

Which enzyme starts at an RNA primer and elongates the new DNA strand?     DNA primase     DNA polymerase III     RNA polymerase     DNA ligase     DNA polymerase I

Below is shown a plasmid map from a recombinant DNA experiment you have performed. You inserted your gene of interest (labeled gene on the map) into the lac Z reporter gene via a Hind III site. If you digested this recombinant DNA with HindIII what is the size of the DNA fragment(s) you would obtain on a DNA gel electrophoresis? The plasmid alone is 4000 bp. I have attached the image below.

Use the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced.

The enzyme is used to remove tension from the DNA double helix during replication called?     Restriction endonuclease   Gyrase   DNA polymerase   Helicase   Ligase

Use the plasmid map for pBashi below to answer the following questions Xhol Hind!!! 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 - HindIII only, Lane 4 - Xhol+ HindIII) 4 kb 3 kb 3 kb 2 kb 1 kb 4 kb Hindill 1 kb 7 kb Xhol Xhol Xhol Xhol Hind!!! Ladder Xhol Hindill Hind!!! Pstl Pstl c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindIII + Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above.…