Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 11, Problem 32P

Alkaptonuria is a human autosomal recessive disorder caused by mutation of the HAO gene that encodes the enzyme homogentisic acid oxidase. Restriction mapping

of the HAO gene region reveals four BamHI restriction sites ( B1 to B4 ) in the wild - type allele and three BamHI restriction sites in the mutant allele. BamHI utilizes the restriction sequence 5'-GGATCC-3' . The BamHI restriction sequence identified as B3 is altered to 5'-GGAACC-3' in the mutant allele. The mutation results in a Ser-to-Thr missense mutation. Restriction maps of the two alleles are shown below, and the binding sites of two molecular probes (probe A and probe B) are identified.

Chapter 11, Problem 32P, Alkaptonuria is a human autosomal recessive disorder caused by mutation of the HAO gene that encodes , example  1

DNA samples taken from a mother (M), father (F), and two children ( C1 and C2 ) are analyzed by Southern blotting of BamHI-digested DNA . The gel electrophoresis results are illustrated.

Chapter 11, Problem 32P, Alkaptonuria is a human autosomal recessive disorder caused by mutation of the HAO gene that encodes , example  2

a. Using A to represent the wild-type allele and a for the mutant allele, identify the genotype of each family member. Identify any family member who is alkaptonuric.

b. In a separate figure, draw the autoradiograph patterns for all the genotypes that could be found in children of this couple.

c. Explain how the DNA sequence change results in a Ser-to-Thr missense mutation.

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Chapter 11 Solutions

Genetic Analysis: An Integrated Approach (3rd Edition)

Ch. 11 - 11.11 Two different mutations are identified in a...Ch. 11 - What is the phenotype effect of inserting a Ds...Ch. 11 - 11.13 Answer the following questions concerning...Ch. 11 - Several types of mutation are identified and...Ch. 11 - 11.15 A sample of the bacterium is exposed to...Ch. 11 - 11.16 A strain of is identified as having a null...Ch. 11 - Describe the difference between DNA transposons...Ch. 11 - 11.18 How are flanking direct repeat sequences...Ch. 11 - 11.19 Using the adeninethymine base pair in this...Ch. 11 - The partial amino acid sequence of a wild-type...Ch. 11 - Prob. 21PCh. 11 - 11.22 Many human genes are known to have homologs...Ch. 11 - The fluctuation test performed by Luria and...Ch. 11 - In this chapter, three features of genes or of DNA...Ch. 11 - Briefly compare the production of DNA double -...Ch. 11 - During mismatch repair, why is it necessary to...Ch. 11 - 11.27 Following the spill of a mixture of...Ch. 11 - 11.28 In an Ames test using Salmonella bacteria a...Ch. 11 - A wild - type culture of haploid yeast is exposed...Ch. 11 - A fragment of a wild - type polypeptide is...Ch. 11 - Prob. 31PCh. 11 - Alkaptonuria is a human autosomal recessive...Ch. 11 - 11.33 In an experiment employing the methods of...Ch. 11 - Using your knowledge of DNA repair pathways choose...Ch. 11 - 11.35 Ataxia telangiectasia is a human inherited...Ch. 11 - A geneticist searching for mutations uses the...Ch. 11 - 11.37 In a mousebreeding experiment a new mutation...Ch. 11 - 11.38 Considering the Dumbo mutation in a Problem,...Ch. 11 - 11.39 Thinking back to the discussion of...Ch. 11 - 11.40 Common baker’s yeast () is normally grown at...Ch. 11 - 11.41 The two gels illustrated below contain...
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