Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 10, Problem 4AQ
Summary Introduction
Reoviridae family of viruses have a wide range of hosts which includes invertebrates, vertebrates, humans, plants and even microbes like
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Some retroviruses that have a wide host range have been developed for use in treatment of diseases or syndromes. These viruses do not result in a productive infection because they lack critical genes for viral
replication, but they do carry DNA sequences that allow them to be packaged into infectious particles. Once in the host cell the viral DNA is released and integrates into the cell's genome. The advantage to this
strategy is that the delivery of the DNA is very efficient. What is one possible problem of using a virus that can integrate into the human nuclear genome?
Multiple Choice
The virus carries genes that will interfere with host cell activities.
The viral DNA sequences could still cause a disease.
Since the viral DNA sequences no longer contain essential genes for viral replication the host cell may not be able to replicate either.
The integration could disrupt an essential gene or genes.
Match each of the following replication classes of viruses with the one description that best
describes it.
This virus class is composed of
segmented and non-segmented
helical viruses that carry an
RdRp within the virion into the
cell. Example: Rabies virus
These viruses are non-
enveloped, icosahedral, have
segmented genomes, and carry
their own RNA-dependent RNA
polymerase. The viral genome
is copied following assembly.
Example: Reovirus
This is the only class of RNA
viruses whose genome is
replicated by cellular enzymes
and not an RNA-dependent
RNA polymerase.
Cellular DNA polymerases
replicate this virus's non-
integrated genome, although
transcription is not the initial
event that takes place in the
process. Example:
Parvoviruses
In this class of viruses,
transcription occurs before
genome replication because
viral proteins are involved in
replicating the genome.
Example: Adenovirus
This class of viruses forms
circular DNA and creates pre-
genomic RNA using host RNA
polymerases.…
After a positive strand RNA virus ( Such as Coronaviruses) enters the host cell, the RNA is translated by ribosomes to create proteins associated with the virus (Such as, for example, the surface antigens like E and Spike proteins). How then, would the genetic material itself be replicated to be incorporated into the new viruses?.
Chapter 10 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 10.1 - Distinguish between a positive-strand RNA virus...Ch. 10.1 - Prob. 2MQCh. 10.1 - Prob. 3MQCh. 10.1 - Describe the classes of viruses based on their...Ch. 10.2 - Prob. 1MQCh. 10.2 - Prob. 2MQCh. 10.2 - Prob. 3MQCh. 10.2 - Prob. 1CRCh. 10.3 - Prob. 1MQCh. 10.3 - In the X174 genome, describe the difference...
Ch. 10.3 - Prob. 3MQCh. 10.3 - Describe how the genome of bacteriophage X174 is...Ch. 10.4 - In what major way does transcription of phage DNA...Ch. 10.4 - Prob. 2MQCh. 10.4 - Why can it be said that transcription of the...Ch. 10.5 - What type of genome is seen in most archaeal...Ch. 10.5 - Compared with other archaeal viruses, what are two...Ch. 10.5 - Prob. 1CRCh. 10.6 - Prob. 1MQCh. 10.6 - Prob. 2MQCh. 10.6 - Prob. 3MQCh. 10.6 - Prob. 1CRCh. 10.7 - Prob. 1MQCh. 10.7 - Prob. 2MQCh. 10.7 - Prob. 3MQCh. 10.7 - Prob. 1CRCh. 10.8 - Prob. 1MQCh. 10.8 - Prob. 2MQCh. 10.8 - How are protein synthesis and genomic replication...Ch. 10.8 - Prob. 1CRCh. 10.9 - Prob. 1MQCh. 10.9 - Prob. 2MQCh. 10.9 - Prob. 3MQCh. 10.9 - Rabies virus and poliovirus both have...Ch. 10.10 - Prob. 1MQCh. 10.10 - Prob. 2MQCh. 10.10 - Prob. 3MQCh. 10.10 - Prob. 1CRCh. 10.11 - Prob. 1MQCh. 10.11 - Prob. 2MQCh. 10.11 - How does the role of reverse transcriptase in the...Ch. 10.11 - Why do both hepadnaviruses and retroviruses...Ch. 10.12 - What type of bacteriophages are most common in the...Ch. 10.12 - Prob. 2MQCh. 10.12 - Prob. 3MQCh. 10.12 - Prob. 1CRCh. 10.13 - Prob. 1MQCh. 10.13 - Prob. 2MQCh. 10.13 - Prob. 3MQCh. 10.13 - Prob. 1CRCh. 10.14 - Prob. 1MQCh. 10.14 - Prob. 2MQCh. 10.14 - Prob. 3MQCh. 10.14 - How do bacterial viruses help prevent human...Ch. 10.15 - If viroids are circular molecules, why are they...Ch. 10.15 - Prob. 2MQCh. 10.15 - Prob. 1CRCh. 10.16 - Prob. 1MQCh. 10.16 - Prob. 2MQCh. 10.16 - Prob. 3MQCh. 10.16 - What are the similarities and differences between...Ch. 10 - Not all proteins are made from the RNA genome of...Ch. 10 - Replication of both strands of DNA in adenoviruses...Ch. 10 - Imagine that you are a researcher at a...Ch. 10 - Prob. 4AQ
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- As a general rule, viruses are not considered to be alive based on several reasons. One such reason is that some viruses use RNA as their genetic material instead of DNA. Which of the five biological themes does this violate? Briefly explain why.arrow_forwardCoronaviruses have a positive-sense single stranded RNA genome. a.) What is the genome replication strategy of this category of viruses? b.) How do you think this feature affects the stability of the viral genome? (hint use figure below) Mutation rates in different types of viruses. Estimates obtained for viruses belonging to the major indicated groups are shown as a function of genome size. This reveals an effect of genetic material (RNA/DNA) and genome size on viral mutation rates.arrow_forwardWhich statement is true of viral replication? A) virus attaches to a specific receptor site on the host-cell membrane. B) Once inside the cell, the viral capsid is degraded, and the viral nucleic acid is released. C) Virus uses the host cell’s machinery to replicate and new virus particles are released. D) All of the abovearrow_forward
- A viral particle adsorbs onto a host cell membrane. It uncoats and releases its single-stranded nonsegmented nucleic acid into the cytoplasm where it is immed dependent RNA polymerase, into mRNA. It is then translated into viral structural proteins and enzymes. Which of the following virus families is most likely repre A) Herpesviridae B) Paramyxoviridae C) Parvoviridae D) Reovirius E) Retroviridaearrow_forwardA particular animal virus requires the use of DNA polymerase from its host, since it does not possess its own DNA polymerase enzyme. Which of the following assumptions in A-D would likely be correct regarding this virus? A) O This virus could not be a retrovirus type. B) OIt could be a (-) ss RNA virus. C) O It could be a (+) ss RNA virus. D) O The vVirus life cycle very likely includes going to the host cell nucleus. E) O All ofA-D are correct assumptions.arrow_forwardThe genome of a retrovirus can integrate into the host cell genome. What gene is unique to retroviruses, and why is the protein encoded by this gene absolutely necessary for maintaining the retroviral life cycle?arrow_forward
- The RNA genomes of some RNA viruses resemble the messenger RNA (mRNA) of their eukaryotic hosts. What advantage would an RNA virus gain by having this type of genome?arrow_forwardViruses are made up of the same biomolecules as cellular organisms (DNA, RNA, proteins, lipids, etc.). They infect cells and replicate themselves using resources from the host cells. Scientists discovered a previously unknown strain of virus in the tropical Pacific Ocean and it can infect higher-order eukaryotes. Initial analysis revealed that the virus is mainly composed of nucleic acids, proteins, and lipids. Chemical analysis after complete hydrolysis revealed the following building blocks (by weight): Chemical components Nucleosides Amino acids Lipids, phosphates, and carbohydrates Percentage by weight 20 30 50 Analysis of percentages of nucleosides yielded the following results (by molar ratio): Nucleosides A G T C U Percentage by mole 19 29 19 29 4 Researchers determined that the lipids found in the virus form a bilayer membrane surrounding the virus, and are obtained from the membrane of the host cell during virus maturation and exit from host cell. Further…arrow_forwardwe can stop the biosynthesis stage of the viral life cycle (not the stages before it) by preventing/inhibiting: A) fusion of the envelope and the plasma membrane B) replication and expression of viral nucleic acid/genome C) the virus's attachment molecules from binding to the host cell receptor D) new copies of viral nucleic acid from combining with newly made viral proteins E)buddingarrow_forward
- A patient has arrived at the doctor complaining of acute respiratory symptoms (cough, runny nose, fever). The patient explains to the doctor that he was at a concert the night before and shared a water bottle with a friend who had similar symptoms. The doctor tells the patient that he has a virus. a.) Can the doctor prescribe an antibiotic for this patient? Explain. b.) What form of replication do you think this virus does use? How do you know? explain in detailsarrow_forwardWhich of the following are the functions of reverse transcriptase in retroviruses? i) it hydrolyzes the host cell's DNA ii) it uses viral RNA as a template for DNA synthesis iii) it converts host cell RNA into viral DNA iv) it translates viral RNA into proteins v) It uses viral RNA as a template for making complementary RNA strand vi) It degrades RNA from DNA-RNA hybrid Oi, iv and v O ii, iv, v and vi ii and iii ii and viarrow_forwardArrange the following list into the correct sequence for part of the cycle of a retrovirus: 1. dsDNA integrated into host DNA 2.viral proteins synthesized on host ribosomes 3. viral DNA uses host enzymes to transcribe viral RNA 4. reverse transcriptase catalyzes synthesis of ssDNA 5. synthesis of second DNA strand (a) 5, 2, 1, 3, 4 (b) 5, 2, 3, 4, 1 (c) 4, 5, 1, 3, 2 (d) 4, 1, 2, 3, 5 (e) 2, 1, 3, 4, 5arrow_forward
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