Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 10.4, Problem 1CR
Why can it be said that transcription of the bacteriophage T7 genome requires two enzymes? Why is bacteriophage Mu mutagenic, and what features are necessary for Mu to insert into DNA?
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Why can it be said that transcription of thebacteriophage T7 genome requires two enzymes?
Considering that prokaryote genomes do not have large introns, how is it possible to move a eukaryotic gene into a transformed bacterium, since they lack a spliceosome?
Suppose a researcher previously cloned gene Y into M13 bacteriophage vector. Gene Y encodes a product called peptide Y. A
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mutant oligonucleotide primer sequence:
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Chapter 10 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 10.1 - Distinguish between a positive-strand RNA virus...Ch. 10.1 - Prob. 2MQCh. 10.1 - Prob. 3MQCh. 10.1 - Describe the classes of viruses based on their...Ch. 10.2 - Prob. 1MQCh. 10.2 - Prob. 2MQCh. 10.2 - Prob. 3MQCh. 10.2 - Prob. 1CRCh. 10.3 - Prob. 1MQCh. 10.3 - In the X174 genome, describe the difference...
Ch. 10.3 - Prob. 3MQCh. 10.3 - Describe how the genome of bacteriophage X174 is...Ch. 10.4 - In what major way does transcription of phage DNA...Ch. 10.4 - Prob. 2MQCh. 10.4 - Why can it be said that transcription of the...Ch. 10.5 - What type of genome is seen in most archaeal...Ch. 10.5 - Compared with other archaeal viruses, what are two...Ch. 10.5 - Prob. 1CRCh. 10.6 - Prob. 1MQCh. 10.6 - Prob. 2MQCh. 10.6 - Prob. 3MQCh. 10.6 - Prob. 1CRCh. 10.7 - Prob. 1MQCh. 10.7 - Prob. 2MQCh. 10.7 - Prob. 3MQCh. 10.7 - Prob. 1CRCh. 10.8 - Prob. 1MQCh. 10.8 - Prob. 2MQCh. 10.8 - How are protein synthesis and genomic replication...Ch. 10.8 - Prob. 1CRCh. 10.9 - Prob. 1MQCh. 10.9 - Prob. 2MQCh. 10.9 - Prob. 3MQCh. 10.9 - Rabies virus and poliovirus both have...Ch. 10.10 - Prob. 1MQCh. 10.10 - Prob. 2MQCh. 10.10 - Prob. 3MQCh. 10.10 - Prob. 1CRCh. 10.11 - Prob. 1MQCh. 10.11 - Prob. 2MQCh. 10.11 - How does the role of reverse transcriptase in the...Ch. 10.11 - Why do both hepadnaviruses and retroviruses...Ch. 10.12 - What type of bacteriophages are most common in the...Ch. 10.12 - Prob. 2MQCh. 10.12 - Prob. 3MQCh. 10.12 - Prob. 1CRCh. 10.13 - Prob. 1MQCh. 10.13 - Prob. 2MQCh. 10.13 - Prob. 3MQCh. 10.13 - Prob. 1CRCh. 10.14 - Prob. 1MQCh. 10.14 - Prob. 2MQCh. 10.14 - Prob. 3MQCh. 10.14 - How do bacterial viruses help prevent human...Ch. 10.15 - If viroids are circular molecules, why are they...Ch. 10.15 - Prob. 2MQCh. 10.15 - Prob. 1CRCh. 10.16 - Prob. 1MQCh. 10.16 - Prob. 2MQCh. 10.16 - Prob. 3MQCh. 10.16 - What are the similarities and differences between...Ch. 10 - Not all proteins are made from the RNA genome of...Ch. 10 - Replication of both strands of DNA in adenoviruses...Ch. 10 - Imagine that you are a researcher at a...Ch. 10 - Prob. 4AQ
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- If an extra nucleotide is inserted in the first exon of the beta globin gene, what effect will it have on the amino acid sequence of the globin polypeptides? Will the globin most likely be fully functional, partly functional, or nonfunctional? Why?arrow_forwardYeast have 8 similar tRNA genes with the anticodon 5’-GUA. A researcher mutates one of these genes to change its anticodon to 5’-GUU. a) What codon did the tRNA originally decode? b) What codon does the mutated tRNA decode?arrow_forwardA certain template DNA strand has the following nucleotide sequence: 3’-TACTCGATGCTGTGCGAT-5’ a) What would be the nucleotide sequence of the complementary nontemplate DNA strand? What is this process called and where does this occur in the eukaryotic cell? b) Take the template strand through the process of transcription. What is the resulting strand called and where does this process occur in a eukaryotic cell. c) Take the template strand through the process of translation including the location in the cell. You should finish with a polypeptide chain. You will need to use the Genetic code found in your notes/bookarrow_forward
- What type of energy is required for new bacteriophages to be assembled?arrow_forwardThe following sequence represents a few codons present in one strand of DNA.Using this strand of DNA as a template strand for transcription, you are required to synthesize a new RNA strand. A) Show the codons that will be present on the RNA strand. B) Using the universal genetic code, provide the amino acids on the protein that will be translated from the RNA strand. 3’ TAC ATG GTT GTG CTA ATT 5’arrow_forwardThe following double stranded segment of DNA is part of a protein coding gene. The segments in uppercase letters (ACTG) represent the exons. The segments in lowercase letters (acgt) represent introns. The lower strand is the template strand that is used by the RNA polymerase to make an RNA transcript. Draw or write-out a) the sequence of the primary transcript and b) the mature mRNA resulting from this stretch of DNA.arrow_forward
- During the process of transcription, the polymerase must handle several distinct polynucleotide chains: the double-stranded DNA ahead of the polymerase, the single-stranded template DNA, the nontemplate DNA strand, and the freshly-synthesized mRNA. How does the polymerase keep each of these strands--as well as the incoming ribonucleotides needed for the polymerase reaction--separate?arrow_forwardWhy might some cells in the body, such as those in bonemarrow, be more susceptible to ribosomal protein mutations than other cell types?arrow_forwardWhy is a trimming process important in converting precursors of tRNA and rRNA to the active forms?arrow_forward
- Yeast have 8 similar tRNA genes with the anticodon 5'-GUA. A researcher mutates one of these genes to change its anticodon to 5'-GUU. a) What codon did the tRNA originally decode? Name the amino acid. b) What codon does the mutated tRNA decode? Name the amino acid.arrow_forwardYou are studying the tryptophan synthetase gene that Yanofsky also examined to determine the relationship between the nucleotide sequence and the amino acid sequence of the gene. Yanofsky found a large number of mutations that affected the tryptophan synthetase gene. A) If you took this mutant E. Coli line (that has an Arginine at this location) and exposed it to a mutagen that could potentially change bases, what are the second mutations you would most likely discover that would restore the activity of the tryptophan synthetase gene and where would it be located? B) Most of the mutations that Yanofsky recovered were missense mutations. However, Yanofsky also recovered a nonsense mutation that changed amino acid number 15 into a stop codon. This codon normally encodes Lysine. Does the recovery of this mutation support the hypothesis that this Lysine residue is critical in the function of the tryptophan synthetase protein?arrow_forwardUsing the transcription unit diagrammed below, in which exons are represented by blue boxes and introns are represented by the connecting lines. You discover a single base deletion in region E of this DNA sequence. Regarding transcription, this mutation will likely: 1.) Result in an alteration to the mRNA sequence. 2.)Have no effect on transcription or the mRNA sequence 3.)Prevent transcription at the TATAA box 4.) Result in an increase or decrease in the amount of mRNA transcribedarrow_forward
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