Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 10, Problem 31ESP
Summary Introduction
To determine: The model of DNA which consistent with the given observations.
Introduction: The analysis of the genetic material of primitive eukaryotes provides some important information. X-ray diffraction experiments show that the DNA of the organism have different dimensions and irregularities. A major hyperchromic shift is also observed on heating and analyzing at 260 nm (UV absorption).
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A primitive eukaryote was discovered that displayed a uniquenucleic acid as its genetic material. Analysis revealed the followingobservations:(a) X-ray diffraction studies display a general pattern similarto DNA, but with somewhat different dimensions and moreirregularity.
(b) A major hyperchromic shift is evident upon heating andmonitoring UV absorption at 260 nm.(c) Base-composition analysis reveals four bases in the followingproportions:Adenine = 8% Hypoxanthine = 18%Guanine = 37% Xanthine = 37%(d) About 75 percent of the sugars are deoxyribose, whereas25 percent are ribose.Attempt to solve the structure of this molecule by postulating amodel that is consistent with the foregoing observations.
Q1)
a) Draw the absorbance spectra for the following DNA samples.
a. Pure DNA without protein contamination
DNA with protein contamination
b.
c. DNA with organic solvent contamination
b) An aqueous sample of genomic DNA gave an optical density of 0.6 AU. The sample was
diluted 1:50 to achieve an OD below 1.0. The measurement was done in a cuvette with a
pathlength of 1 cm. What was the concentration of DNA in the original sample BEFORE
dilution?
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The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your answers, show how you came up to each result?(a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in micrometer?
Chapter 10 Solutions
Concepts of Genetics (12th Edition)
Ch. 10 - Would an experiment similar to that performed by...Ch. 10 - In sea urchin DNA, which is double stranded, 17.5...Ch. 10 - German measles results from an infection of the...Ch. 10 - What vital clues were provided by Franklins work...Ch. 10 - Was it ethical for Wilkins to show Franklins...Ch. 10 - Prob. 3CSCh. 10 - HOW DO WE KNOW? In this chapter, we first focused...Ch. 10 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 10 - Discuss the reasons proteins were generally...Ch. 10 - Contrast the contributions made to an...
Ch. 10 - When Avery and his colleagues had obtained what...Ch. 10 - Why were 32P and 35S chosen for use in the...Ch. 10 - Does the design of the HersheyChase experiment...Ch. 10 - What observations are consistent with the...Ch. 10 - What are the exceptions to the general rule that...Ch. 10 - Draw the chemical structure of the three...Ch. 10 - How are the carbon and nitrogen atoms of the...Ch. 10 - Adenine may also be named 6-amino purine. How...Ch. 10 - Draw the chemical structure of a dinucleotide...Ch. 10 - Describe the various characteristics of the...Ch. 10 - What evidence did Watson and Crick have at their...Ch. 10 - What might Watson and Crick have concluded had...Ch. 10 - How do covalent bonds differ from hydrogen bonds?...Ch. 10 - List three main differences between DNA and RNA.Ch. 10 - What are the three major types of RNA molecules?...Ch. 10 - How is the absorption of ultraviolet light by DNA...Ch. 10 - What is the physical state of DNA after it is...Ch. 10 - What is the hyperchromic effect? How is it...Ch. 10 - Why is Tm related to base composition?Ch. 10 - What is the chemical basis of molecular...Ch. 10 - What did the WatsonCrick model suggest about the...Ch. 10 - A genetics student was asked to draw the chemical...Ch. 10 - Considering the information in this chapter on B-...Ch. 10 - One of the most common spontaneous lesions that...Ch. 10 - In some organisms, cytosine is methylated at...Ch. 10 - Because of its rapid turnaround time, fluorescent...Ch. 10 - Prob. 31ESPCh. 10 - Newsdate: March 1, 2030. A unique creature has...Ch. 10 - During gel electrophoresis, DNA molecules can...Ch. 10 - DNA and RNA are chemically very similar but are...Ch. 10 - Electrophoresis is an extremely useful procedure...
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