Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 10, Problem 1CAE
Summary Introduction
Case summary:
An adult with symptoms of fever, chest pain and cough came to hospital and died within three days due to the wrong treatment without knowing the correct pathogen.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
45-year-old male had been working in his garden when he scratched the knuckle of his right arm during digging in ground with a spade, he immediately washed the area but noticed later that night that the knuckle became red, warm to the touch and swollen. He went to the hospital, the doctor ordered a Gram stain and culture on the pus extracted from the wound. The culture grew the following organism and the Gram stain from the wound showed the following results:Based on the previous results, answer the following questions:1. As a microbiologist, describe what you see on the Gram stain taken from the wound site. And give a preliminary report on what you see on the culture plates. What is the probable Genus and species?2. Name 2 tests that can be done to positively identify this organism.3. What are the media used to culture the sample, and what is the type ofhemolysis seen?
You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies.
How many bacteria (measured in CFU/mL) were in the overnight culture flask?
A. 1.34 x 10^4 CFU/mL
B. 1.34 x 10^5 CFU/mL
C. 1.34 x 10^6 CFU/mL
D. 1.34 x 10^7 CFU/mL
E. 1.34 x 10^8 CFU/mL
F. cannot tell based on the data given - you'd need to know the volume of the original culture flask
Which bacterial culture (Lb. plantarum, P. acidilactici, or P. pentosaceus) was most effective against monocytogenes and Salmonella? Explain why.
What are the limitations of using an agar disk diffusion assay to assess the effectiveness of an antiseptic, disinfectant, or, in this case, a biological control agent on the growth of bacteria of interest?
Chapter 10 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 10 - Which of the following organisms are most closely...Ch. 10 - Here is some additional information on the...Ch. 10 - DRAW IT Use the additional information below to...Ch. 10 - DRAW IT Use the information in the table below to...Ch. 10 - NAME IT Use the key in the Applications of...Ch. 10 - Sergeys Manual of Systematic Bactenoiogy differs...Ch. 10 - Bacillus and Lactobacillus are not in the same...Ch. 10 - Prob. 3MCQCh. 10 - Which of the following is false about scientific...Ch. 10 - You could identify an unknown bacterium by all of...
Ch. 10 - The wall-less mycoplasmas are considered to be...Ch. 10 - Use the following choices to answer questions 7...Ch. 10 - Prob. 8MCQCh. 10 - Prob. 9MCQCh. 10 - Use the following choices to answer questions 9...Ch. 10 - The GC content of Micrococcus is 66-75 moles %,...Ch. 10 - Describe the use of a DNA probe and PCR for: a....Ch. 10 - SF medium is a selective medium, developed in the...Ch. 10 - Prob. 1CAECh. 10 - Prob. 2CAECh. 10 - Prob. 3CAE
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Isolate #2: Cell Morphology (shape) а. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? ***Please answer the four questions base on the picture provided.***arrow_forwardIn lab we learned a technique that helped us to visulize individual colonies of bacter 1. Describe this technique. 2. What do you expect the resutls to look like? Be specific. 3. How can this technique help you to determine if your culture is contaminated? For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS Paragraph I +] F H Ix X ABC † ( O K₂ KN Q V Arial sè "Ω Θ A 4 10pt EE 88 A Click Save and Submit to save and submit. Click Save All Answers to save all answers. 描く前 X² X₂ 3 由用目arrow_forwardA culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): • 60 mL of a bacterial culture is diluted by adding 440 mL of water • 1000 microliters from (A) is added to 9 mL of water • Dilution (B) is used to prepare a 103 dilution in water • 100 ul from dilution (C) is plated to nutrient agar and incubated at 37°C Following incubation, colonies are uniformly distributed on the agar, and 102 colonies are counted on one half of the plate. 1. Express the total CFU/mL of the original culture in scientific notation. 2. If 10 ul is plated from dilution (C), how many colonies should grow on the plate? Edit View Insert Format Tools Tablearrow_forward
- Neosporin consists of neomnycin, bacitracin, and polymyxin B. Which component(s) can inhibit Gram positive bacteria and which can inhibit Gram negative bacteria ? Discuss briefly why it is better to use 3 different antibiotics than just one at the same time.arrow_forwardWhy is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for Splenocyte Preparation?arrow_forwardIs there a relationship between Gram staining and sensitivity to various antibacterial compounds? Please explain.arrow_forward
- Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial drugs? explain your answer?arrow_forwardIsolate #2: Cell Morphology (shape) . a. b. Isolate #2: Gram stain result Gram Postive. (The color I see on my screen is purple.) c. List at least two genera (not species) of bacteria that are consistent with the Gram reaction and morphology you observed. d. Are any of these genera are normal flora on the skin? If so which one(s)? (1 pt) ***Please answer the four questions base on the picture provided.***arrow_forward1. The colonies on a negative MSA plate would appear _____________. 2. "E. Coli and S. epidermidis were chosen to represent Gram-negative and Gram-positive bacteria, respectively. For a given antibiotic, is there a difference in susceptibility between the Gram-positive and Gram-negative bacteria?" "No, they are usually similar" "yes, drugs that target the cell membrane are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram positives" how can I tell the differencearrow_forward
- The disc diffusion assay measuring a disinfectant's effectiveness against a microorganism is more standardized than measuring an antibiotic's effectiveness with the kirby-Bauer assay. a) True b) Falsearrow_forwardWhat is this organism? And what other test could be done to confirm it's identification? 1.Gram stain - positive cocci, chains Catalase - weak positive Hemolysis - beta BE - positive NaCl- positive Bacitracin - no zone of inhibition PYR - positive Answer: Enterococcus spp. CAMP test to verify the identity 2.Gram positive cocci, chains Catalase - negative Hemolysis - alpha BE - negative NaCl - negative P disk - resistant Bile Solubility - negative Answer - viridans group, Use PYR test to verifyarrow_forwardAn elderly man with influenza acquires a case of pneumonia. Gram-positive cocci isolated from his sputum give beta-hemolysis on blood agar. The infection is very difficult to treat. Later, it is shown that the man shared the room with a patient with bone infection. Isolates from both infections were the same. a. Describe 2 biochemical tests that can be used for the definitive diagnosis. b. Describe 2 virulence factors of this pathogen and implicate them in diseases.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
