The disc diffusion assay measuring a disinfectant's effectiveness against a microorganism is more standardized than measuring an antibiotic's effectiveness with the kirby-Bauer assay. a) True b) False
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The disc diffusion assay measuring a disinfectant's effectiveness against a microorganism is more standardized than measuring an antibiotic's effectiveness with the kirby-Bauer assay.
a) True
b) False
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- The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?
- Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolyticWhich of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plateA COVID-19 test, which produce result in 10 minutes, has the following test characteristics. The test has a 95% sensitivity and a 95% specificity. The State of Massachusetts is in dilemma whether to test symptomatic individuals or the entire residents to decrease the spread of infection as depicted in the following two scenarios. Scenario A. The State of Massachusetts wants to administer the test on 40,000 individuals who will develop cough, fever and shortness of breath in the next 10 days to identify COVID-19 infected individuals and institute public health measures. The estimated prevalence of COVID-19 in this population is 750 per 1,000 population using a gold-standard test. Scenario B. The State of Massachusetts wants to administer the test to its entire COVID-19 uninfected population (6,750,000). The estimated prevalence of COVID-19 in this population is 5 per 1,000 population using a gold-standard test. Please help the State of Massachusetts by responding to the…
- Name three reasons why the ELISA test is useful in detecting microbesThe statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?1. You are asked to make 150 ml of 2% agarose using a well-known buffer. How many grams will be used? a) 0.03 g b) 0.3 g c) 3 g d) 30 g 2. Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substrate
- isolating a suspected agent in a sick individual that is absent in a healthy individual is sufficient to establish that the agent causes the disease? True or FalseGive handwritten answer of the all three subparts of the question in imageWhich test require a straight line inoculation? Choose all that apply. A) Bile esculinase B) NaCl C) DNA hydrolysis D) Hemolysin