Why is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for Splenocyte Preparation?

Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the bacteria present in the yogurt sample?

Which among the groups would be affected when the pH is 6.5, and what antibiotic panel will be affected the most. Provide two (2) corrective actions to resolve this error.

Novobiocin susceptibility: Zone of inhibition or no zone of inhibition present: ______________ Novobiocin Susceptible or resistant: ______________________

How can I explain the results of my findings in lamen terms? 1 - Name of Test Type of Medium 2 - Color Before Incubation 3-Color After Incubation? 4 – Results Positive/ Negative? 5 - Reagents Added Lactose Fermentation test Phenol Red Lactose (PRL) Red Color: Yellow   Gas Bubble: Yes Positive Phenol Red to detect color change (added) Glucose Fermentation Test Phenol Red Glucose (PRG) Red Color: Yellow   Gas Bubble: Yes Positive Phenol Red to detect color change (added) Citrate Utilization Simmons Citrate Green Color: Blue Positive Bromthymol Blue (added) Indole Test Media = SIM Clear Indole: Color: Clear Negative For Indole, we add Kovac’s   Hydrogen Sulfide Test Media = SIM clear Hydrogen Sulfide: Color: Clear Negative Iron already added Methyl Red Test (MR test) Media = MRVP Broth Clear Color: Yellow Negative We add Methyl Red Reagent Voges-Proskauer Test (VP test) Media = MRVP Broth Clear…

Which bacterial culture (Lb. plantarum, P. acidilactici, or P. pentosaceus) was most effective against monocytogenes and Salmonella? Explain why. What are the limitations of using an agar disk diffusion assay to assess the effectiveness of an antiseptic, disinfectant, or, in this case, a biological control agent on the growth of bacteria of interest?

List the ingredients and recipe (in gram or milligram) to make one liter (1000 ml) of Trypticase Soy Agar, nutrient agar, and Columbia blood medium (use a table

In growth promotion test, TYMC in one plate is 150 cfu. Interpretation: Rationale:

Vibrio cholerae can be cultured on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Enterobacteriaceae and gram-positive bacteria are inhibited on this agar. cholerae produces large yellow colonies while other Vibrio species produce blue, green, or blue-green colonies. Discuss the purpose of each media listed. Based on this information alone, what term(s) would best describe this agar? Selective Enrichment (enriched) Differential Reducing Selective and enriched Selective and differential Selective, differential, and enriched