What is this organism? And what other test could be done to confirm it's identification? 1.Gram stain - positive cocci, chains Catalase - weak positive Hemolysis - beta BE - positive NaCl- positive Bacitracin - no zone of inhibition PYR - positive Answer: Enterococcus spp. CAMP test to verify the identity 2.Gram positive cocci, chains Catalase - negative Hemolysis - alpha BE - negative NaCl - negative P disk - resistant Bile Solubility - negative Answer - viridans group, Use PYR test to verify
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What is this organism? And what other test could be done to confirm it's identification?
1.
Catalase - weak positive
Hemolysis - beta
BE - positive
NaCl- positive
Bacitracin - no zone of inhibition
PYR - positive
Answer: Enterococcus spp. CAMP test to verify the identity
2.Gram positive cocci, chains
Catalase - negative
Hemolysis - alpha
BE - negative
NaCl - negative
P disk - resistant
Bile Solubility - negative
Answer - viridans group, Use PYR test to verify
Step by step
Solved in 2 steps
- From a contaminated intravenous catheter you isolate Gram-positive cocci, beta-hemolytic, and catalase positive. MSA results below (grown aerobically): Which pathogen from the list below have you isolated? O Streptococcus pyogenes_ O Staphylococcus aureus_ Staphylococcus epidermidis_ Streptococcus pneumoniae_I AM TRYING TO IDENTIFY THIS UNKNOWN. ***IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. ***IMAGE 2 HAS TWO TESTS CONDUCTED ON IT, BACITRACIN AND PYR I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which one of the above is thge unknown. You have pictures of the unkown on Catalase and Blood Agar and three test conducted on Bacitracin and PYR. Give reasoning as why you think it will be one of the above. Explaing the characteristics and what made you deicide it?Gram classification Etiologic agent Drug (s) of choice Vaccines (if applicable) Gram-positive coci Streptococcus hemolytic, groups A.B,C,G Viridans streptococci Gram-positive coci Gram-positive coci Staphylococcus, methicillin-resistant Gram-positive cocci Staphylococcus, non- penicillinase producing Escherichia coli (UTI) Enteric Gram- negative rods Enteric Gram- negative rods Klebsiella pneumoniae Enteric Gram- negative rods Serratia marcescens Enteric Gram- negative rods Proteus vulgaris 米 Enteric Gram- Salmonella typhi negative rods O Focu
- Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus) CAMP Test Coagulase Tube Test PYR Test Novobiocin Sensitivity TestI AM TRYING TO IDENTIFY THIS UNKNOWN. ***IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. ***IMAGE 2 HAS THREE TESTS CONDUCTED ON IT, OXIDASE, BACITRACIN AND NOVOBIOCIN. I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which one of the above is thge unknown. You have pictures of the unkown on Catalase and Blood Agar and three test conducted on Oxidase, Bacitracin and Novobiocin. Give reasoning as why you think it will be one of the above. Explaing the characteristics and what made you deicide it?I tested organism growth on the MSA plate for strep. pyongenes, strep. pneumonia, s. aureus, and straph. epidermidis and my results were that streptococci organism did not grow or change color and the s.aureus changed color (yellow) and s.epi there was no change in color but they both showed growth. 1. If an unknown gram-positive bacterium was given, what would it mean if it grew on MSA? Name the organisms used above My answer: that it would be either s.aureus or s. epidermis because they are selective for salt-tolerant bacteria and can grow on mannitol 2. what would it mean if it both grew on MSA and turned yellow? Can you add to my answer or correct it. Thank you!
- I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which test will i need out of the table to justify your reason of picking up the unknown and also how that test justifies it? what characteristics of that test made you pick the unknown?Why do Salmonella growing on XLT-4 agar is red colony with black center. Explain it in detail and concisely.A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?
- The smear prepared from the gram-positive control shown gram-negative results. Describe 3 reasons why this incorrect reaction could occur Can the possession of flagella by bacteria be utilized for classification purposes? Explain.Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 XYou are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flask