You have a drug that blocks the activity of ligase. Describe what effect this drug would have on replication of the leading strand. Explain your reasoning in one sentence or less.

If ligase was defective in a cell, which strand of DNA Replication do you think would be most affected, the leading or lagging strand? Fully explain why you think that by describing the enzyme function and the differences in replication in the leading and lagging strands.

Explain why a higher agarose concentration is preferred when seperating smaller DNA molecules. Include mathematical analysis in your answer.

Use the gel diagram below to answer Questions 62 and 63. The positively-charged electrode of the electrophoresis gel is shown at the bottom of the diagram; the negatively-charged electrode is at the top. The mixture in each well included the original DNA fragment, all four dNTPs, one type of ddNTP as labeled in the diagram, and other necessary components of the dideoxy chain-termination reaction. The results are below. ddATP ddGTP ddCTP ddTTP (A) (B) (C) (D) (E) +. 62. Which of the following represents the sequence of the template strand?" A. 5'--GAAGACTAACATTCA--3' B. 5'-- ACTTACAATGTAGUA--3' C. 5'-- CTTCTGATTGTAAGT--3' D. 5'--ACTTACAATCAGAAG--3' E. 5'--TGAATGTTAGTCTTC--3'

What is the enzyme used to create DNA complementary to mature mRNA? Group of answer choices ligase topoisomerase DNA polymerase reverse transcriptase RNA polymerase transposase

Give typing answer with explanation and conclusion

Can you please answer number 9 and all of the sub parts

Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…

Which of the following has activity that is most similar to RNA Polymerase?     Helicase     DNA Polymerase     Primase     Exonuclease

Which process does NOT use a specific nucleotide sequence for activity? please explain the answer a.replication initiation b.DNA helicase unwinding c.transcriptional termination d.translational termination